Study of the polysaccharide production by the microalgae C-1509 Nannochloris sp. Naumann

Olga Babich; Svetlana Ivanova; Aleksandr Tupitsyn; Aleksandr Vladimirov; Elena Nikolaeva; Archana Tiwari; Ekaterina Budenkova; Egor Kashirskikh; Veronika Anokhova; Philippe Michaud; Stanislav Sukhikh

doi:10.1016/j.btre.2023.e00818

Study of the polysaccharide production by the microalgae C-1509 Nannochloris sp. Naumann

Biotechnol Rep (Amst). 2023 Oct 30:40:e00818. doi: 10.1016/j.btre.2023.e00818. eCollection 2023 Dec.

Authors

Affiliations

¹ Research and Education Center, Industrial Biotechnologies, Immanuel Kant BFU, A. Nevsky Street, 14, Kaliningrad, Russia.
² Natural Nutraceutical Biotesting Laboratory, Kemerovo State University, Krasnaya Street 6, Kemerovo 650043, Russia.
³ Department of TNSMD Theory and Methods, Kemerovo State University, Krasnaya Street, 6, Kemerovo 650043, Russia.
⁴ Laboratory of Carbon Nanomaterials, R&D Department, Kemerovo State University, Krasnaya Street, 6, Kemerovo 650043, Russia.
⁵ P.A. Chikhachev House of Scientific Collaboration, Kemerovo State University, Krasnaya Street, 6, Kemerovo 650043, Russia.
⁶ Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida 201301, India.
⁷ Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, Clermont-Ferrand F-63000, France.

Abstract

Biologically active compounds, including polysaccharides isolated from microalgae, have various properties. Although Nannochloropsis spp. have the potential to produce secondary metabolites important for biotechnology, only a small part of the research on these microalgae has focused on their ability to produce polysaccharide fractions. This study aims to evaluate the physicochemical growth factors of Nannochloropsis spp. microalgae, which ensure the maximum accumulation of polysaccharides, as well as to optimize the parameters of polysaccharide extraction. The optimal nutrient medium composition was selected to maximize biomass and polysaccharide accumulation. The significance of selecting the extraction module and extraction temperature regime, as well as the cultivation conditions (temperature and active acidity value) is emphasized. Important chemical components of polysaccharides responsible for their biological activity were identified.

Keywords: Biological properties; Biomass; Nannochloropsis spp.; Nutrient medium; Physicochemical growth factors; Polysaccharides.