Precision-Cut Slice Culture Method for Rat Placenta

Fusun Gundogan; Jeffrey Gilligan; Suzanne de la Monte

doi:10.1007/978-1-0716-3495-0_4

Precision-Cut Slice Culture Method for Rat Placenta

Methods Mol Biol. 2024:2728:35-41. doi: 10.1007/978-1-0716-3495-0_4.

Authors

Fusun Gundogan^{1

2}, Jeffrey Gilligan³, Suzanne de la Monte^{4

5

6}

Affiliations

¹ Regional Perinatal Pathology, Kaiser Permanente Oakland Medical Center, Oakland, CA, USA. fusun.x.gundogan@kp.org.
² Department of Pathology and Laboratory Medicine, The Warren Alpert Medical School of Brown University, Providence, RI, USA. fusun.x.gundogan@kp.org.
³ Department of Neurosurgery, Mount Sinai Health System, New York, NY, USA.
⁴ Regional Perinatal Pathology, Kaiser Permanente Oakland Medical Center, Oakland, CA, USA.
⁵ Department of Pathology and Laboratory Medicine, The Warren Alpert Medical School of Brown University, Providence, RI, USA.
⁶ Department of Pathology and Laboratory Medicine, Rhode Island Hospital, Providence, RI, USA.

PMID: 38019390
DOI: 10.1007/978-1-0716-3495-0_4

Abstract

Organotypic slice cultures provide several advantages over primary and cell line culture models. Unlike monolayer cultures, organotypic slice cultures retain the tissue's 3-D architecture, multi-lineage cell populations, and many complex functional characteristics. Organotypic slice cultures can be exposed to biological, pharmacological, or environmental agents and analyzed to predict in vivo responses. Herein, we describe a method for preparing organotypic slice cultures generated from rat placenta.

Keywords: Methodology; Organotypic; Placenta; Slice culture; Trophoblast.

MeSH terms

Animals
Cell Culture Techniques*
Cell Line
Culture Techniques
Female
Microphysiological Systems*
Placenta
Pregnancy
Rats