Universal PCR for bacteria, mycobacteria, and fungi: a 10-year retrospective review of clinical indications and patient outcomes

J Clin Microbiol. 2023 Dec 19;61(12):e0095223. doi: 10.1128/jcm.00952-23. Epub 2023 Nov 28.

Abstract

Our work provides a retrospective analysis of universal PCR orders for bacteria, mycobacteria, and fungi across our institution across a 10-year period. We assessed the positivity rates for this diagnostic tool by test type and specimen type and, critically, studied whether and how the results influenced the outcomes from treatment change, to readmission, to death.

Keywords: laboratory utilization; molecular diagnostics; outcomes; universal PCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fungi* / genetics
  • Humans
  • Mycobacterium* / genetics
  • Polymerase Chain Reaction / methods
  • Retrospective Studies