Single-cell RNA sequencing reveals immune microenvironment of small cell lung cancer-associated malignant pleural effusion

Thorac Cancer. 2024 Jan;15(1):98-103. doi: 10.1111/1759-7714.15145. Epub 2023 Nov 27.

Abstract

We used 10 × genomics single-cell transcriptome sequencing technology to reveal the tumor immune microenvironment characteristics of small cell lung cancer (SCLC) in a patient with malignant pleural effusion (MPE). A total of 8008 high-quality cells were finally obtained for subsequent bioinformatic analysis, which were divided into 10 cell clusters further identified as B cells, T cells, myeloid cells, NK cells, and cancer cells. Such SCLC related genes as NOTCH1, MYC, TSC22D1, SOX4, BLNK, YBX3, VIM, CD8A, CD8B, and KLF6 were expressed in different degrees during differentiation of T and B cells. Different ligands and receptors between T, B and tumor cells almost interact through MHC II, IL-16, galectin, and APP signaling pathway.

Keywords: immune microenvironment; malignant pleural effusion; single-cell transcriptome sequencing; small cell lung cancer.

Publication types

  • Case Reports

MeSH terms

  • Cell Differentiation
  • Humans
  • Lung Neoplasms* / pathology
  • Pleural Effusion*
  • Pleural Effusion, Malignant* / genetics
  • Pleural Effusion, Malignant* / pathology
  • SOXC Transcription Factors / genetics
  • Sequence Analysis, RNA
  • Small Cell Lung Carcinoma* / complications
  • Small Cell Lung Carcinoma* / genetics
  • Tumor Microenvironment / genetics

Substances

  • SOX4 protein, human
  • SOXC Transcription Factors