Single-cell RNA sequencing to understand host-virus interactions

Jia-Tong Chang; Li-Bo Liu; Pei-Gang Wang; Jing An

doi:10.1016/j.virs.2023.11.009

Single-cell RNA sequencing to understand host-virus interactions

Virol Sin. 2024 Feb;39(1):1-8. doi: 10.1016/j.virs.2023.11.009. Epub 2023 Nov 25.

Authors

Jia-Tong Chang¹, Li-Bo Liu¹, Pei-Gang Wang², Jing An³

Affiliations

¹ Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.
² Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China. Electronic address: pgwang@ccmu.edu.cn.
³ Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China. Electronic address: anjing@ccmu.edu.cn.

Abstract

Single-cell RNA sequencing (scRNA-seq) has allowed for the profiling of host and virus transcripts and host-virus interactions at single-cell resolution. This review summarizes the existing scRNA-seq technologies together with their strengths and weaknesses. The applications of scRNA-seq in various virological studies are discussed in depth, which broaden the understanding of the immune atlas, host-virus interactions, and immune repertoire. scRNA-seq can be widely used for virology in the near future to better understand the pathogenic mechanisms and discover more effective therapeutic strategies.

Keywords: COVID-19; Flavivirus; Host-virus interaction; Single-cell RNA sequencing (scRNA-seq).

Publication types

Review

MeSH terms

Gene Expression Profiling*
Host Microbial Interactions* / genetics
Sequence Analysis, RNA