Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

Silvanir; Sin Yuan Lai; Azren Aida Asmawi; Kit Wayne Chew; Cheng Loong Ngan

doi:10.1016/j.biortech.2023.130094

Application of high shear-assisted liquid biphasic system for protein extraction from Chlorella sp

Bioresour Technol. 2024 Feb:393:130094. doi: 10.1016/j.biortech.2023.130094. Epub 2023 Nov 22.

Authors

Silvanir¹, Sin Yuan Lai², Azren Aida Asmawi³, Kit Wayne Chew⁴, Cheng Loong Ngan⁵

Affiliations

¹ School of Energy and Chemical Engineering, Xiamen University Malaysia, Sepang, Selangor Darul Ehsan 43900, Malaysia.
² School of Energy and Chemical Engineering, Xiamen University Malaysia, Sepang, Selangor Darul Ehsan 43900, Malaysia; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China.
³ Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Gambang 26300, Pahang Darul Makmur, Malaysia.
⁴ School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, 637459, Singapore.
⁵ School of Energy and Chemical Engineering, Xiamen University Malaysia, Sepang, Selangor Darul Ehsan 43900, Malaysia; College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, Fujian, China. Electronic address: clngan@xmu.edu.my.

PMID: 38000640
DOI: 10.1016/j.biortech.2023.130094

Abstract

Microalgae is a sustainable alternative source to traditional proteins. Existing pretreatment methods for protein extraction from microalgae still lack scalability, are uneconomical and inefficient. Herein, high shear mixing (HSM) was applied to disrupt the rigid cell walls and was found to assist in protein release from microalgae. This study integrates HSM in liquid biphasic system with seven parameters being investigated on extraction efficiency (EE) and protein yield (Y). The highest EE and Y obtained are 96.83 ± 0.47 % and 40.98 ± 1.27 %, respectively, using 30% w/v K₃PO₄ salt, 60 % v/v alcohol, volume ratio of 1:1 and 0.5 % w/v biomass loading under shearing rate of 16,000 rpm for 1 min.

Keywords: Aqueous two-phase system; Biorefinery; Chlorella vulgaris; Rotor-stator homogenization; Separation.

MeSH terms

Biomass
Cell Wall
Chlorella vulgaris*
Chlorella* / metabolism
Microalgae* / metabolism