Ultrafast spectroscopic studies on the interaction of reactive oxygen species with a probe impregnated in nanoscopic and microscopic matrix formulation

Lopamudra Roy; Nivedita Pan; Susmita Mondal; Ria Ghosh; Md Nur Hasan; Neha Bhattacharyya; Soumendra Singh; Kallol Bhattacharyya; Arpita Chattopadhyay; Samir Kumar Pal

doi:10.1016/j.saa.2023.123671

Ultrafast spectroscopic studies on the interaction of reactive oxygen species with a probe impregnated in nanoscopic and microscopic matrix formulation

Spectrochim Acta A Mol Biomol Spectrosc. 2024 Mar 5:308:123671. doi: 10.1016/j.saa.2023.123671. Epub 2023 Nov 20.

Affiliations

¹ Department of Applied Optics and Photonics, JD-2, Sector-III, Salt Lake, Kolkata 700 106, India.
² Department of Chemical and Biological Sciences, S. N. Bose National Centre for Basic Sciences, Kolkata 700 106, India.
³ Department of Radio Physics and Electronics, University of Calcutta, 92, Acharya Prafulla Chandra Rd, Machuabazar, Kolkata 700009, India.
⁴ Department of Basic Science and Humanities, Techno International New Town, Block - DG 1/2 New Town, Action Area 1, Kolkata 700156, India. Electronic address: arpita.chattopadhyay@tict.edu.in.
⁵ Department of Chemical and Biological Sciences, S. N. Bose National Centre for Basic Sciences, Kolkata 700 106, India. Electronic address: skpal@bose.res.in.

PMID: 38000325
DOI: 10.1016/j.saa.2023.123671

Abstract

Reactive oxygen species (ROS) plays important role to maintain homeostasis in living bodies. Here we have studied interaction of ROS generated from hydrogen peroxide (H₂O₂) with a well-known spectroscopic probe Rose Bengal (RB) encapsulated in nanoscopic sodium dodecyl sulphate (SDS) micelles in aqueous medium and entrapped in microscopic nylon 66 solid matrix generated using electrospinning technique. A detailed spectroscopic characterization of ROS with SDS encapsulated RB (RB-SDS) shows efficient interaction compared to that in bulk medium. The time resolved analysis on the probe based on femtosecond resolved 2D-spectrum time images collected from streak camera reveal the simultaneous existence of an ultrafast electron (∼6 ps) and a hole transfer mechanism (∼93 ps) resulting from generation of hydroxyl radicals through photobleaching of the probe in presence of H₂O₂. Based on the spectroscopic and time resolved studies of RB in bulk and in restricted (SDS) medium, we have further translated it for the development of an in-field prototype device which utilizes RB as a ROS sensor impregnated in a nylon thin film. The microscopic nylon solid matrix characterized by scanning electron microscope (SEM) shows porous structure for holding sample containing ROS. Our study quantitatively measures the amount of ROS by using RB embedded microfiber membrane. Thus, our developed prototype device based on RB embedded on the nylon matrix would be beneficial for the potential use in quantification of ROS in extracellular fluids and food materials.

Keywords: Femto-second resolved ultrafast spectroscopy; Nylon matrix; Prototype; Reactive Oxygen Species; Rose Bengal; SDS.