How we do it: the Zurich Microsurgery Lab technique for placenta preparation

Acta Neurochir (Wien). 2023 Dec;165(12):3821-3824. doi: 10.1007/s00701-023-05847-5. Epub 2023 Nov 23.

Abstract

Background: Perfused placentas provide an excellent and accessible model for microvascular dissection, microsuturing and microanastomosis training - particularly in the early microsurgical learning curve. This way, a significant amount of live animals can be spared.

Method: We present the Zurich Microsurgery Lab protocol, detailing steps for obtaining, selecting, cleaning, flushing, cannulating, and preserving human placentas - as well as microsurgical training examples - in a tried-and-true, safe, cost-effective, and high-yield fashion.

Conclusion: Our technique enables highly realistic microsurgical training (microdissection, microvascular repair, microanastomosis) based on readily available materials. Proper handling, preparation, and preservation of the perfused placenta models is key.

Keywords: Education; Microanastomosis; Microsurgery; Microsurgical training; Microsuturing; Microvascular anastomosis; Microvascular surgery; Placenta.

MeSH terms

  • Anastomosis, Surgical / methods
  • Animals
  • Clinical Competence
  • Dissection
  • Female
  • Humans
  • Microdissection
  • Microsurgery* / methods
  • Placenta* / blood supply
  • Placenta* / surgery
  • Pregnancy