More than 25% of all known plant viruses are transmitted through seeds, which makes this mode of dispersal of great importance for plant virus epidemics. Virus detection in seed stocks remains the most frequent approach for seed health testing, but current methods are not always standardized and/or do not allow analyzing large numbers of seeds. Here, we describe a high-throughput method to quantify plant virus seed transmission rate based on classical grow-out tests, which can be applied to widely different viruses and host species.
Keywords: Arabidopsis thaliana; Grow-out test; Plant virus; RT-qPCR virus detection; Seed transmission.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.