Agroinoculation of Geminiviral Infectious Clones into Plants

Abstract

Most geminiviruses are not transmitted by mechanical inoculation. Therefore, pathogenicity and plant-pathogen interaction studies rely on agroinoculation using infectious clones, which involves cloning the geminiviral genome in a binary vector (see previous chapter for details). A suspension containing the infectious clone inserted into Agrobacterium tumefaciens cells is then inoculated into plants, i.e., agroinoculated. Below is a simple protocol for agroinoculation of an infectious geminivirus clone into plants.

Keywords: Agrobacterium radiobacter; Agrobacterium tumefaciens; Agroinjection; Geminivirus; Infectious clone inoculation.