Antimicrobial properties of Limosilactobacillus reuteri strains for control of Escherichia coli and Salmonella strains, diarrhoea cause in weaning pigs

Yoonjeong Yoo; Jihwan Lee; Jinho Cho; Yohan Yoon

doi:10.17221/112/2022-VETMED

Antimicrobial properties of Limosilactobacillus reuteri strains for control of Escherichia coli and Salmonella strains, diarrhoea cause in weaning pigs

Vet Med (Praha). 2023 May 29;68(5):191-199. doi: 10.17221/112/2022-VETMED. eCollection 2023 May.

Authors

Yoonjeong Yoo¹, Jihwan Lee², Jinho Cho³, Yohan Yoon^{1

4}

Affiliations

¹ Department of Food and Nutrition, Sookmyung Women's University, Seoul, Republic of Korea.
² Department of Poultry Science, University of Georgia, Athens, GA, USA.
³ Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea.
⁴ Risk Analysis Research Center, Sookmyung Women's University, Seoul, Republic of Korea.

Abstract

This study aimed to use lactic acid bacteria isolated from piglet faeces to develop probiotics, allowing for the effective control of Escherichia coli and Salmonella. Lactic acid bacteria were isolated from the faeces of suckling piglets and identified by 16S rRNA sequencing, then examined for haemolysis; gelatinase activity; and resistance to acid, bile, and pancreatin. The antimicrobial activity of selected lactic acid bacteria isolates was examined for 8 E. coli and 7 Salmonella strains. One-hundred and sixty-four lactic acid bacteria isolates were identified from 118 piglet faecal samples, and 13 lactic acid bacteria isolates were selected from analyses of haemolysis; gelatinase activity; and resistance to acid, bile, and pancreatin. Of the selected 13 lactic acid bacteria isolates, Limosilactobacillus reuteri PF20-3 and PF30-3 strains had the highest antibacterial activity against E. coli and Salmonella.

Keywords: antibiotic resistance; feed additive; gut health; gut microbiota; probiotics.

Grants and funding

Supported by grants from the Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (Z-1543081-2020-22-0103).