Immobilizing glucose oxidase on AuCu hydrogels for enhanced electrochromic biosensing

Yanan Chen; Lei Jiao; Ruimin Li; Lijun Hu; Xiangkun Jia; Zhijun Zhu; Yanling Zhai; Xiaoquan Lu

doi:10.1016/j.aca.2023.341977

Immobilizing glucose oxidase on AuCu hydrogels for enhanced electrochromic biosensing

Anal Chim Acta. 2023 Dec 1:1283:341977. doi: 10.1016/j.aca.2023.341977. Epub 2023 Oct 28.

Authors

Yanan Chen¹, Lei Jiao², Ruimin Li¹, Lijun Hu¹, Xiangkun Jia¹, Zhijun Zhu³, Yanling Zhai⁴, Xiaoquan Lu⁵

Affiliations

¹ Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China.
² Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China. Electronic address: jiaolei@qdu.edu.cn.
³ Institute of Hybrid Materials, National Center of International Research for Hybrid Materials Technology, National Base of International Science & Technology Cooperation, College of Materials Science and Engineering, Qingdao University, Qingdao, 266071, PR China. Electronic address: zhuzhijun@qdu.edu.cn.
⁴ Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China. Electronic address: zhaiyanling@qdu.edu.cn.
⁵ Institute of Molecular Metrology, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao, 266071, PR China. Electronic address: luxq@nwnu.edu.cn.

PMID: 37977794
DOI: 10.1016/j.aca.2023.341977

Abstract

Development of highly sensitive and accurate biosensors still faces a great challenge. Herein, glucose oxidase (GOx) is efficiently immobilized on the AuCu hydrogels owing to their porous structure and interfacial interaction, demonstrating enhanced catalytic activity, satisfactory stability and recyclability. Besides, by integration of AuCu@GOx and electrochromic material of Prussian blue, a sensitive and stable biosensing platform based on the excellent electrochromic property of Prussian blue and the enhanced enzyme activity of AuCu@GOx is developed, which enables the electrochemical and visual dual-mode detection of glucose. The as-constructed biosensing platform possesses a wide linear range, and good selectivity for glucose detection with a limit of detection of 0.82 μM in visual mode and 0.84 μM in electrochemical mode. This easy-to-operate biosensing platform opens a door for the practical application of the multi-mode strategy for glucose detection.

Keywords: Electrochromism; Enzyme immobilization; Glucose detection; Noble metal hydrogels.

MeSH terms

Biosensing Techniques*
Enzymes, Immobilized / chemistry
Ferrocyanides
Glucose
Glucose Oxidase* / chemistry

Substances

Glucose Oxidase
ferric ferrocyanide
Enzymes, Immobilized
Glucose
Ferrocyanides