Biological synthesis of nanoparticles is cost-effective as well as safer than physical and chemical methods. This study focuses on the biological synthesis of silver nanoparticles using Glutamicibacter uratoxydans which remains still unexplored. The synthesized silver nanoparticles are encapsulated with chitosan to prepare nanobiocomposite. Actinobacteria were isolated from mesophilic soil and screened for heavy metal resistance. The potent heavy metal resistant isolate was identified by 16SrRNA sequencing and used for the biological synthesis of silver particles. The characterization of chitosan- silver nano-bio composite was carried out by UV-Vis spectroscopy, FTIR spectroscopy, and XRD. Morphology was analyzed by scanning electron microscopy. The particle size and stability were studied using Dynamic light scattering and Zeta potential analysis. The nano-bio composite was tested for lead removal efficiency and antibiofilm activity. The potent isolate was identified as Glutamicibacter uratoxydans and it was named as Glutamicibacter uratoxydans VRAK 24. The UV spectra showed maximum absorbance at 410 nm. The FTIR spectra and XRD confirmed chitosan encapsulation with silver nanoparticle. The size of nanobiocomposite was found to be 0.376. The stability of nanobiocomposite recorded a zeta potential value of -5.37 mV. The lead removal efficiency was found to be 87.69 %. In addition, the nanobiocomposite exhibited highest anti-biofilm activity against S.aureus when compared to E.coli. The research findings, concluded that the synthesized nanobiocomposite showed better anti-biofilm activity. Also, nanobiocomposite was found to be a good adsorbent for the removal of heavy metal lead.
Keywords: Chitosan nano-bio composite; Glutamicibacter uratoxydans VRAK 24; Lead removal efficiency.
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