Heterogeneity of leaf stoichiometry of different life forms along environmental transects in typical ecologically fragile areas of China

Abstract

The coupling between carbon (C):nitrogen (N):phosphorus (P) stoichiometry in plant leaves is closely related to ecological functions such as photosynthesis, growth, and biogeochemical cycling. To explore the biogeographic patterns, nutrient limitations, and the relationships between leaf and soil stoichiometry, as well as the factors influencing leaf stoichiometry, we quantified community-level leaf C:N:P stoichiometry in trees, shrubs, and herbs along transects with a total length of about 4300 km. The leaf C:N:P ratios of trees, shrubs, and herbs were approximately 349:13:1, 267:14:1, and 226:12:1, respectively. Leaf C:N:P stoichiometry differed significantly (p < 0.05) among the life forms. Compared with global and Chinese scales, the C, N, and P concentrations were higher and C:N, C:P, and N:P ratios were lower. The leaf C:N:P stoichiometry patterns along a latitude gradient differed among life forms. There was no significant correlation between leaf N and soil total N, whereas leaf P of all three life forms increased significantly with increasing soil total P. Those results suggested a community-level N limitation for trees, shrubs, and herbs growth. Environmental factors explained 43.9, 26.5, and 6.1 % of leaf stoichiometric variations for trees, shrubs, and herbs, respectively. However, the key environmental driving factors gradually changed from climatic factors for trees and shrubs to soil factors for herbs. The results provide new insights into community-level biogeographical patterns and potential factors of leaf stoichiometry among plant life forms.

Keywords: Ecological fragile area; Latitude pattern; Leaf C:N:P stoichiometry; Life form; Nutrient limitation.