In vivo antioxidant and hypoglycaemic potentials of Rivina humilis extract against streptozotocin induced diabetes and its complications in wistar rats

Ramesh C; Kaushik Ghosh; Sowmya B A; Pinkey Rawal; Soma Pramanik

doi:10.1007/s40200-023-01258-6

In vivo antioxidant and hypoglycaemic potentials of Rivina humilis extract against streptozotocin induced diabetes and its complications in wistar rats

J Diabetes Metab Disord. 2023 Aug 17;22(2):1373-1383. doi: 10.1007/s40200-023-01258-6. eCollection 2023 Dec.

Authors

Ramesh C¹, Kaushik Ghosh¹, Sowmya B A¹, Pinkey Rawal², Soma Pramanik²

Affiliations

¹ Department of Pharmacology, East West College of Pharmacy, No-63, I Bharath Nagar, Off Magadi Road Vishwaneedam (PO), Bangalore, 560091 India.
² Department of Pharmaceutical Chemistry, East West College of Pharmacy, Bangalore, India.

PMID: 37975104
PMCID: PMC10638325 (available on 2024-08-17)
DOI: 10.1007/s40200-023-01258-6

Abstract

Purpose: This current research study was designed to investigate beneficial effects of R. humilis (Rivina humilis) against streptozotocin-induced diabetic rats.

Methods: The R. humilis ethanol extract was prepared using soxhlet and its phenol content was determined. The type-2 diabetes was induced in rats by giving fructose mixed drinking water and single dose of streptozotocin. Oral glucose tolerance test (OGTT) was performed after 72 h of streptozotocin to check ability of extract to utilize oral glucose load with 2 h. The extract was also tested for its potentials to reduce blood glucose (BGL) and diabetic complications by administering to diabetic rats for 21 days. Blood glucose was determined on day 1, 7, 14 and 21. At 21st day, blood samples were collected from experimental rats were euthanized to collect pancreas and liver. Liver and kidney function tests, HbAc1 and lipid profile was established from blood samples. Pancreas was subjected to histopathological examination and liver was used to determine antioxidant enzymes. In vitro study was done to investigate the effect of extract on glucose utilization by rat hemidiaphragm.

Results: In OGTT, administration of extract could stimulate glucose utilization which was witnessed by significant BGL reduction at 90 and 120 min in therapeutic groups compare to diabetics. In chronic study, we observed significant reduction in BGL on 21st day and all tests performed to determine liver and kidney function, HbAc1, vitamin E were normal in extract treated groups. There was significant increase in liver antioxidant enzymes in therapeutic groups which revealed regeneration of β-cells in therapeutic groups.

Conclusion: The results of research demonstrated significant antidiabetic potentials in R. humilis.

Supplementary information: The online version contains supplementary material available at 10.1007/s40200-023-01258-6.

Keywords: ALT; AST; Anti-oxidant; Hypoglycaemic; Lipid profile; Rivina humilis and streptozotocin; Serum glucose; Urea.

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