Application of Diffusive Gradients in Thin-films (DGT) for assessing the heavy metals mobility in soil and prediction of their transfer to Russula virescens

Abstract

Although edible mushrooms are considered a source of many beneficial nutrients for human, they can also represent a risk to health due to their capacity to accumulate heavy metals. In this study, the total dissolved in soil solution and labile concentrations of heavy metals (Cd, Pb, Cu, Zn, Co, Cr, Mn, Ni, and Fe) in soil were measured and correlated with their concentrations accumulated in Russula virescens wild mushrooms. The diffusive gradient in thin films (DGT) technique was used to measure the labile metals content in the soil (C_DGT), and corroborated with the metals concentrations in soil solution (C_soln) was used to calculate an R-value, which can estimate the metals resupply from soil solid phase when they are uptake by mushroom. The DGT-labile metal concentrations decreased in the order Mn > Fe > Zn > Cu > Co > Ni > Cd ≅ Pb > Cr. The R-values, calculated as the ratio between C_DGT and C_soln decreased in the order: Cd (0.50) > Zn (0.37) > Pb (0.33) > Cu (0.24) ≅ Ni (0.24) ≅ Co (0.23) > Mn (0.16) > Fe (0.12) > Cr (0.04). For the first time, we compared the R-values with the bioaccumulation factors (BAFs) in mushrooms, and it was observed that, a similar increasing trend of BAFs with the R-values exists, thus the capacity of the soil solid phase to fast re-supply metals to soil solution increases BAFs. Although the soil samples were not contaminated with heavy metals above the legislative limits, the concentrations of heavy metals accumulated in mushrooms were high enough to pose risks for humans, mainly for children, due to their Cu content.

Keywords: Bioavailability; DGT; Health risk; Heavy metals; Transfer factors; Wild mushrooms.