Highly Selective Colorimetric Sensor of Mercury(II) Ions by Andrographolide-Stabilized Silver Nanoparticles in Water and Antibacterial Evaluation

Chanon Talodthaisong; Pitiphoom Sangiamkittikul; Panupong Chongwichai; Apichart Saenchoopa; Saengrawee Thammawithan; Rina Patramanon; Suppanat Kosolwattana; Sirinan Kulchat

doi:10.1021/acsomega.3c03789

Highly Selective Colorimetric Sensor of Mercury(II) Ions by Andrographolide-Stabilized Silver Nanoparticles in Water and Antibacterial Evaluation

ACS Omega. 2023 Oct 23;8(44):41134-41144. doi: 10.1021/acsomega.3c03789. eCollection 2023 Nov 7.

Authors

Affiliations

¹ Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
² Materials Chemistry Research Center, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
³ Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.

Abstract

Silver nanoparticles (AgNPs) are well known for their exceptional properties and versatility in various applications. This study used andrographolide as a biochemical stabilizer to synthesize AgNPs (andro-AgNPs). The andro-AgNPs were characterized by using UV-vis spectroscopy, revealing a surface plasmon resonance peak at 440 nm. Fourier transform infrared spectroscopy was also used to confirm the presence of AgNPs. Transmission electron microscopy was used to investigate the morphology of andro-AgNPs, which showed a spherical shape with an average diameter of 18.30 ± 5.57 nm (n = 205). Andro-AgNPs were utilized as a colorimetric sensor to detect mercury ions (Hg²⁺) in water, and the optimized detection conditions were evaluated using UV-vis spectroscopy with a linear range of 15-120 μM. The limit of detection and the limit of quantification for Hg²⁺ detection were found to be 11.15 and 37.15 μM, respectively. Furthermore, andro-AgNPs exhibited antibacterial properties against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. The results imply that andro-AgNPs hold promising potential for future biomedical applications.