AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

Xiaohui Bai; Kun Zhang; Chaopeng Ou; Yanyu Mu; Dongmei Chi; Jianxing Zhang; Jingxiu Huang; Xile Li; Yingjun Zhang; Wan Huang; Handong Ouyang

doi:10.1016/j.isci.2023.108227

AKAP150 from nucleus accumbens dopamine D1 and D2 receptor-expressing medium spiny neurons regulates morphine withdrawal

iScience. 2023 Oct 17;26(11):108227. doi: 10.1016/j.isci.2023.108227. eCollection 2023 Nov 17.

Authors

Xiaohui Bai^{1

2}, Kun Zhang¹, Chaopeng Ou¹, Yanyu Mu¹, Dongmei Chi¹, Jianxing Zhang¹, Jingxiu Huang¹, Xile Li¹, Yingjun Zhang¹, Wan Huang¹, Handong Ouyang¹

Affiliations

¹ Department of Anesthesiology, State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-sen University Cancer Center, Guangzhou, P.R. China.
² Department of Anesthesiology, Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China.

Abstract

Dopamine D1 receptor-expressing medium spiny neurons (D1R-MSNs) and dopamine D2 receptor-expressing MSNs (D2R-MSNs) in nucleus accumbens (NAc) have been demonstrated to show different effects on reward and memory of abstinence. A-kinase anchoring protein 150 (AKAP150) expression in NAc is significantly upregulated and contributes to the morphine withdrawal behavior. However, the underlying mechanism of AKAP150 under opioid withdrawal remains unclear. In this study, AKAP150 expression in NAc is upregulated in naloxone-precipitated morphine withdrawal model, and knockdown of AKAP150 alleviates morphine withdrawal somatic signs and improves the performance of conditioned place aversion (CPA) test. AKAP150 in NAc D1R-MSNs is related to modulation of the performance of morphine withdrawal CPA test, while AKAP150 in NAc D2R-MSNs is relevant to the severity of somatic responses. Our results suggest that AKAP150 from D1R-MSNs or D2R-MSNs in NAc contributes to the developmental process of morphine withdrawal but plays different roles in aspects of behavior or psychology.

Keywords: Behavioral neuroscience; Biological sciences; Natural sciences; Neuroscience; Systems neuroscience.