Synergistic co-catalytic nanozyme system for highly efficient one-pot colorimetric sensing at neutral pH: Combining molybdenum trioxide and Fe(III)-Modified covalent triazine framework

Jingyan Xu; Hanying Tan; Xionghui Ma; Linjing Su; Zhi Zhang; Yuhao Xiong

doi:10.1016/j.ab.2023.115391

Synergistic co-catalytic nanozyme system for highly efficient one-pot colorimetric sensing at neutral pH: Combining molybdenum trioxide and Fe(III)-Modified covalent triazine framework

Anal Biochem. 2024 Jan 15:685:115391. doi: 10.1016/j.ab.2023.115391. Epub 2023 Nov 11.

Authors

Jingyan Xu¹, Hanying Tan², Xionghui Ma³, Linjing Su⁴, Zhi Zhang², Yuhao Xiong⁵

Affiliations

¹ School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, PR China; Guangxi Key Laboratory of Food Science and Technology, College of Food and Bioengineering, Hezhou University, Hezhou, 542899, PR China.
² Guangxi Key Laboratory of Food Science and Technology, College of Food and Bioengineering, Hezhou University, Hezhou, 542899, PR China.
³ Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs Haikou, 571101, PR China.
⁴ Guangxi Key Laboratory of Food Science and Technology, College of Food and Bioengineering, Hezhou University, Hezhou, 542899, PR China. Electronic address: linjingsu@163.com.
⁵ School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, PR China; Guangxi Key Laboratory of Food Science and Technology, College of Food and Bioengineering, Hezhou University, Hezhou, 542899, PR China. Electronic address: xiongyuhao@yeah.net.

PMID: 37952895
DOI: 10.1016/j.ab.2023.115391

Abstract

This study investigates the co-catalytic capabilities of MoO₃ nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The unique physicochemical surface properties and two-dimensional structures of Fe-CTF enable the direct immobilization of native enzymes (glucose oxidase (GOD) and xanthine oxidase (XOD)) through adsorption, eliminating the need for chemical processes. Efficient immobilization of the native enzymes within the Fe-CTF/GOD(XOD) hybrid is achieved through multipoint attachment involving various interactions. The Fe-CTF/MoO₃ co-catalytic system exhibits enzyme-mimicking activity at neutral pH and, when combined with the high catalytic activity of the immobilized native enzymes, enables the development of a colorimetric method for glucose detection. This method demonstrates excellent facilitation, rapidity, sensitivity, and selectivity, with a linear detection range of 50-1000 μM and a limit of detection of 8.8 μM for glucose. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors. The inhibitory potential of a crude extract derived from Chinese water chestnut peel on XOD activity is evaluated using this method. The findings of this study pave the way for the utilization of nanozyme/native enzyme hybrids in pH-neutral conditions for one-pot colorimetric sensing. This work contributes to the advancement of enzyme-based sensing technologies and holds promise for various applications in biosensing and biomedical research.

Keywords: Colorimetric sensing; Fe(III)-Modified covalent triazine framework; MoO(3) nanosheets; Neutral pH enzyme-like activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colorimetry* / methods
Ferric Compounds* / chemistry
Glucose
Glucose Oxidase / chemistry
Hydrogen Peroxide
Hydrogen-Ion Concentration
Triazines

Substances

molybdenum trioxide
Ferric Compounds
Triazines
Glucose
Glucose Oxidase
Hydrogen Peroxide