VH-CH1 switch region-inserting multispecific antibody designs and their efficacy against SARS-CoV-2 in vitro and in vivo

Affiliations

• ¹ CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
• ² College of Life Science, Hebei University, Baoding, Hebei, China.
• ³ Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, and KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
• ⁴ Kunming National High-level Biosafety Research Center for Non-Human Primates, Center for Biosafety Mega-Science, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
• ⁵ Cryo-EM Center, Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong, China.
• ⁶ Institute of Physical Science and Information, Anhui University, Hefei, Anhui, China.
• ⁷ Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, and KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China. yaoyg@mail.kiz.ac.cn.
• ⁸ Kunming National High-level Biosafety Research Center for Non-Human Primates, Center for Biosafety Mega-Science, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China. yaoyg@mail.kiz.ac.cn.
• ⁹ University of the Chinese Academy of Sciences, Beijing, China. yaoyg@mail.kiz.ac.cn.
• ¹⁰ National Resource Center for Non-Human Primates, National Research Facility for Phenotypic & Genetic Analysis of Model Animals (Primate Facility), Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China. yaoyg@mail.kiz.ac.cn.
• ¹¹ CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. gaof@im.ac.cn.
• ¹² University of the Chinese Academy of Sciences, Beijing, China. gaof@im.ac.cn.
• ¹³ Chinese Center for Disease Control and Prevention, Beijing, China. gaof@im.ac.cn.
• ¹⁴ CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China. wangqihui@im.ac.cn.
• ¹⁵ College of Life Science, Hebei University, Baoding, Hebei, China. wangqihui@im.ac.cn.
• ¹⁶ Institute of Physical Science and Information, Anhui University, Hefei, Anhui, China. wangqihui@im.ac.cn.
• ¹⁷ University of the Chinese Academy of Sciences, Beijing, China. wangqihui@im.ac.cn.