β-Glucosidase-producing microbial community in composting: Response to different carbon metabolic pressure influenced by biochar

Abstract

Poor management of agricultural waste will cause a lot of environment pollution and the composting process is one of the most effective measures for resource reuse of agricultural waste. β-Glucosidase-producing microbial communities play a vital role in cellulose degradation during composting and regulate cellulase production via differentially expressed glucose/non-glucose tolerant β-glucosidase genes. Biochar is widely used as an amendment in compost to accelerate cellulose degradation during composting. However, Biochar-mediated impacts on β-glucosidase-producing microbial communities in compost are unclear. Here, different carbon metabolism pressures were set in natural and biochar compost to elucidate the regulation mechanism and interaction of the β-glucosidase microbial community. Results showed that the addition of biochar decreased the transcription of β-glucosidase genes and led to a reduction of β-glucosidase activity. Micromonospora and Cellulosimicrobium were the predominant functional communities determining cellulose degradation during biochar compost. Biochar addition strengthened the response of the functional microbial community to carbon metabolism pressure. And adding biochar altered the key β-glucosidase-producing microbial communities, influencing cellulase and the interaction between these communities to respond to the different carbon metabolic pressure of compost. Biochar also shifted the co-occurrence network of β-glucosidase-producing microbial community by changing the keystone species. Furthermore, co-occurrence network analysis revealed that high glucose decreased the complexity and stability of the functional microbial network. Most functional microorganisms from Streptomyces produce non-glucose tolerant β-glucosidase, which were the key bacterial communities affecting β-glucosidase activity in the non-glucose treatment. This study provides new insights into the response of functional microbial communities and the regulation of enzyme production during the transformation of cellulosic biomass.

Keywords: Biochar; Compost; Functional microbial community; Glucose tolerant; β-glucosidase.