Generation and characterization of PDGFRα-GFP knock-in mice for visualization of PDGFRα+ fibroblasts in vivo

Xueying Yang; Xufeng Li; Zhihou Guo; Zhuonan Zhang; Xin Song; Mingjun Zhang; Ximeng Han; Lingjuan He; Bin Zhou

doi:10.1016/j.bbrc.2023.149215

Generation and characterization of PDGFRα-GFP knock-in mice for visualization of PDGFRα⁺ fibroblasts in vivo

Biochem Biophys Res Commun. 2023 Dec 20:687:149215. doi: 10.1016/j.bbrc.2023.149215. Epub 2023 Nov 4.

Authors

Xueying Yang¹, Xufeng Li¹, Zhihou Guo², Zhuonan Zhang², Xin Song², Mingjun Zhang³, Ximeng Han³, Lingjuan He⁴, Bin Zhou⁵

Affiliations

¹ Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China.
² School of Life Sciences, Westlake University, Hangzhou, Zhejiang, 310030, China.
³ New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China.
⁴ School of Life Sciences, Westlake University, Hangzhou, Zhejiang, 310030, China. Electronic address: helingjuan@westlake.edu.cn.
⁵ Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China; New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China. Electronic address: zhoubin@sibs.ac.cn.

PMID: 37949027
DOI: 10.1016/j.bbrc.2023.149215

Abstract

The platelet-derived growth factor (PDGF) and its receptor, PDGFRα, are critical for tissue development and injury repair. To track PDGFRα-expressing cells in vivo, we generated a knock-in mouse line that expresses green fluorescent protein (GFP) under the control of the PDGFRα promoter. This genetic tool enabled us to detect PDGFRα expression in various organs during both neonatal and adult stages. Additionally, we confirmed the correlation between endogenous PDGFRα and transgenic PDGFRα expression using mouse injury models, showing the potential of this genetic reporter for studying PDGFRα-mediated signaling pathways and developing therapeutic strategies. Overall, the PDGFRα-GFP knock-in mouse line serves as a valuable tool for investigating the biology of PDGFRα and its role in normal development and disease.

Keywords: Fibroblasts; In vivo visualization; PDGFRα-GFP; Tissue regeneration.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Disease Models, Animal
Fibroblasts* / metabolism
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Mice
Mice, Transgenic
Receptor, Platelet-Derived Growth Factor alpha* / genetics
Receptor, Platelet-Derived Growth Factor alpha* / metabolism

Substances

Receptor, Platelet-Derived Growth Factor alpha
Green Fluorescent Proteins