Transcription factor CncC regulates the expression of antennal CYP6MU1 gene responsible for trans-2-hexen-1-al and nonanal recognition in Locusta migratoria

Haihua Wu; Huichao Wang; Ruiying Li; Yongmei Liu; Yichao Zhang; Nan Chen; Weina Kong; Fei Zhao; Xueyao Zhang; Jianzhen Zhang

doi:10.1016/j.pestbp.2023.105620

Transcription factor CncC regulates the expression of antennal CYP6MU1 gene responsible for trans-2-hexen-1-al and nonanal recognition in Locusta migratoria

Pestic Biochem Physiol. 2023 Nov:196:105620. doi: 10.1016/j.pestbp.2023.105620. Epub 2023 Sep 14.

Authors

Haihua Wu¹, Huichao Wang², Ruiying Li², Yongmei Liu², Yichao Zhang¹, Nan Chen³, Weina Kong⁴, Fei Zhao⁴, Xueyao Zhang⁵, Jianzhen Zhang⁶

Affiliations

¹ Institute of Applied Biology, Shanxi Key Laboratory of Nucleic Acid Biopesticides, Shanxi University, Taiyuan 030006, Shanxi, PR China.
² Institute of Applied Biology, Shanxi Key Laboratory of Nucleic Acid Biopesticides, Shanxi University, Taiyuan 030006, Shanxi, PR China; School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, PR China.
³ Guangmeiyuan R&D Center, Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology, South China Normal University, Meizhou 514779, Guangdong, PR China.
⁴ College of Plant Protection, Shanxi Agricultural University, Taiyuan 030031, Shanxi, PR China.
⁵ Institute of Applied Biology, Shanxi Key Laboratory of Nucleic Acid Biopesticides, Shanxi University, Taiyuan 030006, Shanxi, PR China. Electronic address: zxy@sxu.edu.cn.
⁶ Institute of Applied Biology, Shanxi Key Laboratory of Nucleic Acid Biopesticides, Shanxi University, Taiyuan 030006, Shanxi, PR China. Electronic address: zjz@sxu.edu.cn.

PMID: 37945256
DOI: 10.1016/j.pestbp.2023.105620

Abstract

Cytochrome P450 monooxygenases (P450s) are a superfamily of multifunctional heme-containing proteins and could function as odorant-degrading enzymes (ODEs) in insect olfactory systems. In our previous study, we identified a P450 gene from the antennal transcriptome of Locusta migratoria, LmCYP6MU1, which could be induced by a variety of volatiles. However, the regulatory mechanisms of this gene in response to volatiles remain unknown. In current study, we investigated the tissues and development stages expression patterns of LmCYP6MU1 and determined its olfactory function in the recognition of the main host plant volatiles which induced LmCYP6MU1 expression. The results showed that LmCYP6MU1 was antenna-rich and highly expressed throughout the antennal developmental stages of locusts. LmCYP6MU1 played important roles in the recognition of trans-2-hexen-1-al and nonanal. Insect CncC regulates the expression of P450 genes. We tested whether LmCncC regulates LmCYP6MU1 expression. It was found that LmCncC knockdown in the antennae resulted in the downregulation of LmCYP6MU1 and repressed the volatiles-mediated induction of LmCYP6MU1. LmCncC knockdown reduced the electroantennogram (EAG) and behavioral responses of locusts to volatiles. These results suggested that LmCncC could regulate the basal and volatiles-mediated inducible expression of LmCYP6MU1 responsible for the recognition of trans-2-hexen-1-al and nonanal. These findings provide an original basis for understanding the regulation mechanisms of LmCncC on LmCYP6MU1 expression and help us better understand the LmCncC-mediated olfactory plasticity.

Keywords: Host plant volatiles; LmCYP6MU1; LmCncC; Locusta migratoria; Olfaction; Regulation.

MeSH terms

Animals
Arthropod Antennae / metabolism
Gene Expression Regulation
Insect Proteins / genetics
Insect Proteins / metabolism
Locusta migratoria* / genetics
Locusta migratoria* / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism
Transcriptome

Substances

nonanal
Transcription Factors
Insect Proteins