Identification and antibiogram of Enterobacterales from direct urine samples using matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) technology and disk-plate diffusion technique

Jorge Jover-García; Celia López-Millán; Jesús J Gil-Tomás; Manuel Callejón-Fernández; María Lecuona-Fernández

doi:10.1016/j.ijmmb.2023.100457

Identification and antibiogram of Enterobacterales from direct urine samples using matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) technology and disk-plate diffusion technique

Indian J Med Microbiol. 2023 Nov-Dec:46:100457. doi: 10.1016/j.ijmmb.2023.100457. Epub 2023 Aug 10.

Authors

Jorge Jover-García¹, Celia López-Millán², Jesús J Gil-Tomás³, Manuel Callejón-Fernández⁴, María Lecuona-Fernández⁵

Affiliations

¹ Microbiology Service, Hospital Universitario de Canarias, Carretera Ofra s/n, 38320, La Cuesta, Santa Cruz de Tenerife, Canary Islands, Spain. Electronic address: kokejover@gmail.com.
² Microbiology Service, Hospital Universitario de Canarias, Carretera Ofra s/n, 38320, La Cuesta, Santa Cruz de Tenerife, Canary Islands, Spain. Electronic address: celialm89@gmail.com.
³ Laboratory Clinical Management Unit, Hospital La Inmaculada, Av. Dra. Ana Parra s/n, 04600, Huércal-Overa, Almería, Spain. Electronic address: jesus.j.gil@uv.es.
⁴ Microbiology Service, Hospital Universitario de Canarias, Carretera Ofra s/n, 38320, La Cuesta, Santa Cruz de Tenerife, Canary Islands, Spain. Electronic address: macafer4@gmail.com.
⁵ Microbiology Service, Hospital Universitario de Canarias, Carretera Ofra s/n, 38320, La Cuesta, Santa Cruz de Tenerife, Canary Islands, Spain. Electronic address: mlecuona2005@yahoo.es.

PMID: 37945131
DOI: 10.1016/j.ijmmb.2023.100457

Abstract

Identification and antibiogram of uropathogenic microorganisms from direct urine samples present a great clinical impact. Here, we present a combined procedure to determine identification (IDd) of bacteria through MALDI-TOF-MS technology and antibiogram (ATBd) using disk-plate diffusion technique, of UTI-producing Enterobacterales against the most used antibiotics. Ninety-four urine samples with presence of pyuria and Gram-negative bacilli were selected. The IDd showed a high success rate (90%). ATBd procedure showed a high correlation for tested antibiotics. This simplified, low cost and reduced work time two-step procedure significantly reduces results turnaround time and benefit the clinical management of patients with UTI.

Keywords: Antibiogram; MALDI-TOF-MS; Urinary tract infection; Urosepsis.

MeSH terms

Anti-Bacterial Agents / pharmacology
Humans
Lasers
Microbial Sensitivity Tests
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Urinary Tract Infections* / diagnosis
Urinary Tract Infections* / drug therapy
Urinary Tract Infections* / microbiology

Substances

Anti-Bacterial Agents