Isolation, Identification and Screening of Saharan Actinomycete Strain Streptomyces fimbriatus AC31 Endowed with Antimicrobial Activity

Omar Khirennas; Slimane Mokrani; Belkacem Behira; Noureddine Bouras; El Hadj Driche; Ouahiba Moumen

doi:10.22088/IJMCM.BUMS.12.1.51

Isolation, Identification and Screening of Saharan Actinomycete Strain Streptomyces fimbriatus AC31 Endowed with Antimicrobial Activity

Int J Mol Cell Med. 2023;12(1):51-69. doi: 10.22088/IJMCM.BUMS.12.1.51.

Authors

Omar Khirennas^{1

2}, Slimane Mokrani^{3

4}, Belkacem Behira^{1

2}, Noureddine Bouras^{5

6}, El Hadj Driche^{5

7}, Ouahiba Moumen²

Affiliations

¹ Laboratory of Geo-Environment and Spatial Development (LGEDE), Faculty of Nature and Life Sciences, University Mustapha Stambouli of Mascara, Mascara, Algeria.
² Laboratory of Geomatics, Ecology and Environment (LGO2E), Faculty of Nature and Life Sciences, University Mustapha Stambouli of Mascara, Mascara, Algeria.
³ Laboratory of Research on Biological Systems and Geomatics, Faculty of Nature and Life Sciences, University Mustapha Stambouli of Mascara, Mascara, Algeria.
⁴ Laboratory of Renewable Energies Management, Faculty of Natural and Life Sciences, University of Bejaia, Algeria.
⁵ Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
⁶ Laboratoire de Valorisation et Conservation des Ecosystèmes Arides (LVCEA), Université de Ghardaia, Ghardaïa, Algeria.
⁷ Laboratoire de Biologie Moléculaire, Génomique et Bio-Informatique (LBMGB), Faculté des Sciences de la Nature et de la Vie (SNV), Université Hassiba Benbouali de Chlef, Hay Salem, 02000 Chlef, Algeria.

Abstract

The increasing global public health concern of antimicrobial resistance (AMR) necessitates exploration of natural antimicrobial agents as potential alternatives. This study aimed to investigate antimicrobial activities of Saharan actinomycetes, with specific focus on the strain Streptomyces fimbriatus AC31, that holds promising potential as an alternative to combat AMR. In this context, 32 actinomycetes were isolated from El Atteuf (Ghardaïa), Algeria. Isolates obtained were characterized morphologically and biochemically. Screened isolate was identified by 16S rRNA gene sequencing. Classification of actinomycete isolates was carried out by UPGMA (Unweighted Pair Group Method with Arithmetic Mean). Then, they were screened for their antimicrobial activity by cross-streak method. Identification of 32 isolates revealed 5 genera: Streptomyces (65.63%), Nocardia (9.38%), Streptosporangium (9.38%), Nocardiopsis (9.38%) and Actinomadura (6.25%). According to the biochemical and physiological characteristics, UPGMA classified the isolates in 4 phenons. A number of 24 (75.00%) isolates were active against Gram-positive bacteria, 21 (65.63%) isolates were effective against Gram-negative bacteria, and 25 (78.13%) isolates inhibited Candida albicans. Screened strain Streptomyces fimbriatus AC31 showed highest antagonistic activity and revealed an inhibition zones of 41, 38, 41, 42, and 44 mm, against B. subtilis (ATCC 6633), E. coli (ATCC 8739), S. typhimurium (ATCC 13331), S. aureus (ATCC 6538) and C. albicans (ATCC 10231), respectively. Phylogenetic identification of the AC 31 isolate using 16S rRNA gene sequence showed similarity of 100% with Streptomyces fimbriatus NBRC 15411^T. Actinomycete isolates characterized in this study were endowed with antimicrobial activity against various pathogenic microorganisms that could be used efficiently in developing new antimicrobial substances.

Keywords: Actinomycetes; Saharan soil; antimicrobial activity; screening.