Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish

Jie Sun; Qiuxin Yan; Zhihao Zhang; Ting Xu; Ye Gong; Weijia Li; Kangsen Mai; Qinghui Ai

doi:10.1016/j.isci.2023.108207

Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish

iScience. 2023 Oct 13;26(11):108207. doi: 10.1016/j.isci.2023.108207. eCollection 2023 Nov 17.

Authors

Jie Sun¹, Qiuxin Yan¹, Zhihao Zhang¹, Ting Xu¹, Ye Gong¹, Weijia Li¹, Kangsen Mai^{1

2}, Qinghui Ai^{1

2}

Affiliations

¹ Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
² Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao, Shandong 266237, People's Republic of China.

Abstract

Chromatin remodeling plays an important role in regulating gene transcription, in which chromatin remodeling complex is a crucial aspect. Brg1/Brm-associated factor 60c (BAF60c) subunit forms a bridge between chromatin remodeling complexes and transcription factors in mammals; hence, it has received extensive attention. However, the roles of BAF60c in fish remain largely unexplored. In this study, we identified BAF60c-interacting proteins by using HIS-pull-down and LC-MS/MS analysis in fish. Subsequently, the RNA-seq analysis was performed to identify the overall effects of BAF60c. Then, the function of BAF60c was verified through BAF60c knockdown and overexpression experiments. We demonstrated for the first time that BAF60c interacts with glucose-regulated protein 78 (GRP78) and regulates lipid metabolism, endoplasmic reticulum (ER) stress, and inflammation. Knockdown of BAF60c reduces fatty acid biosynthesis, ER stress, and inflammation. In conclusion, the results enriched BAF60c-interacting protein network and explored the function of BAF60c in lipid metabolism and inflammation in fish.

Keywords: Biological sciences; Molecular biology; Physiology; Zoology.