Serum mannan-binding lectin-associated serine proteases in early pregnancy for gestational diabetes in Chinese pregnant women

Ming Gao; Jing Li; Rui Zhang; Ninghua Li; Weiqin Li; Shuang Zhang; Peng Wang; Hui Wang; Zhongze Fang; Zhijie Yu; Gang Hu; Junhong Leng; Xilin Yang

doi:10.3389/fendo.2023.1230244

Serum mannan-binding lectin-associated serine proteases in early pregnancy for gestational diabetes in Chinese pregnant women

Front Endocrinol (Lausanne). 2023 Oct 24:14:1230244. doi: 10.3389/fendo.2023.1230244. eCollection 2023.

Authors

Ming Gao^#¹, Jing Li^#^{1

2

3}, Rui Zhang¹, Ninghua Li¹, Weiqin Li⁴, Shuang Zhang⁴, Peng Wang⁴, Hui Wang¹, Zhongze Fang⁵, Zhijie Yu⁶, Gang Hu⁷, Junhong Leng⁴, Xilin Yang^{1

2

3}

Affiliations

¹ Department of Epidemiology and Biostatistics, School of Public Health, Tianjin Medical University, Tianjin, China.
² Tianjin Key Laboratory of Environment, Nutrition and Public Health, Tianjin Medical University School of Public Health, Tianjin, China.
³ Tianjin Center for International Collaborative Research on Environment, Nutrition and Public Health, Tianjin Medical University School of Public Health, Tianjin, China.
⁴ Project Office, Tianjin Women and Children's Health Center, Tianjin, China.
⁵ Department of Toxicology and Sanitary Chemistry, School of Public Health, Tianjin Medical University, Tianjin, China.
⁶ Population Cancer Research Program and Department of Pediatrics, Dalhousie University, Halifax, NS, Canada.
⁷ Chronic Disease Epidemiology Laboratory, Pennington Biomedical Research Center, Baton Rouge, LA, United States.

^# Contributed equally.

Abstract

Aims: This study aimed to explore associations of mannan-binding lectin-associated serine protease (MASP) levels in early pregnancy with gestational diabetes mellitus (GDM). We also examined interactions of MASPs and deoxycholic acid (DCA)/glycoursodeoxycholic acid (GUDCA) for the GDM risk and whether the interactive effects if any on the GDM risk were mediated via lysophosphatidylcholine (LPC) 18:0.

Materials and methods: A 1:1 case-control study (n = 414) nested in a prospective cohort of pregnant women was conducted in Tianjin, China. Binary conditional logistic regressions were performed to examine associations of MASPs with the GDM risk. Additive interaction measures were used to examine interactions between MASPs and DCA/GUDCA for the GDM risk. Mediation analyses and Sobel tests were used to examine mediation effects of LPC18:0 between the copresence of MASPs and DCA/GUDCA on the GDM risk.

Results: High MASP-2 was independently associated with GDM [odds ratio (OR): 2.62, 95% confidence interval (CI): 1.44-4.77], while the effect of high MASP-1 on GDM was attributable to high MASP-2 (P for Sobel test: 0.003). Low DCA markedly increased the OR of high MASP-2 alone from 2.53 (1.10-5.85) up to 10.6 (4.22-26.4), with a significant additive interaction. In addition, high LPC18:0 played a significant mediating role in the links from low DCA to GDM and from the copresence of high MASP-2 and low DCA to GDM (P for Sobel test <0.001) but not in the link from high MASP-2 to GDM.

Conclusions: High MASP-1 and MASP-2 in early pregnancy were associated with GDM in Chinese pregnant women. MASP-2 amplifies the risk of low DCA for GDM, which is mediated via LPC18:0.

Keywords: deoxycholic acid; gestational diabetes mellitus; glycoursodeoxycholic acid; lysophosphatidylcholines; mannan-binding lectin-associated serine proteases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Case-Control Studies
Diabetes, Gestational* / epidemiology
East Asian People
Female
Humans
Mannose-Binding Lectin*
Mannose-Binding Protein-Associated Serine Proteases / analysis
Pregnancy
Pregnant Women
Prospective Studies

Substances

Mannose-Binding Protein-Associated Serine Proteases
Mannose-Binding Lectin

Grants and funding

This research was supported by the National Natural Science Foundation of China (Grant No: 82200932) and Tianjin Key Medical Discipline (Specialty) Construction Project (Grant No: TJYXZDXK- 075C).