Streptomyces changanensis sp. nov. Isolated from Soil in China

Abstract

An aerobic, Gram-positive, and non-motile actinomycete, designated HL-66 ^T, was isolated from a soil sample collected in the Meridian Valley, Shaanxi Province, China. Morphological, chemotaxonomic, and phylogenetic characteristics showed a high similarity to the genus Streptomyces. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbour of HL-66 ^T were Streptomyces lavendofoliae NBRC 12882 ^T (99.17%), Streptomyces gobitricini NBRC 15419 ^T (99.03%) and Streptomyces roseolilacinus NBRC 12815 ^T (98.96%). Genome relatedness indexes revealed that the average nucleotide identity and digital DNA-DNA hybridization values between HL-66 ^T and its closest phylogenomic relative (S. roseolilacinus JCM 4335 ^T) were 88.61% and 32.10%, respectively. The cell-wall peptidoglycan contains _LL-diaminopimelic acid. Predominant menaquinones are MK-9 (H₆), MK-9(H₄) and MK-9(H₈). The major cellular fatty acids were iso-C_16:0, anteiso-C_15:0, iso-C_16:1 H, and C_16:1 ω7c. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, and an unknown phospholipid. Based on phylogenetic analyses, genome-genome distance calculation, and average nucleotide identity, strain HL-66 ^T represents a novel species of the genus Streptomyces. Therefore, a new species Streptomyces changanensis sp. nov. is proposed with strain HL-66 ^T (= CGMCC 22674 = JCM 35800) as the type strain.