Single-cell RNA sequencing-guided fate-mapping toolkit delineates the contribution of yolk sac erythro-myeloid progenitors

Y X Zhao; J Y Song; X W Bao; J L Zhang; J C Wu; L Y Wang; C He; W Shao; X L Bai; T B Liang; J P Sheng

doi:10.1016/j.celrep.2023.113364

Single-cell RNA sequencing-guided fate-mapping toolkit delineates the contribution of yolk sac erythro-myeloid progenitors

Cell Rep. 2023 Nov 28;42(11):113364. doi: 10.1016/j.celrep.2023.113364. Epub 2023 Nov 3.

Authors

Y X Zhao¹, J Y Song¹, X W Bao², J L Zhang¹, J C Wu¹, L Y Wang³, C He⁴, W Shao⁵, X L Bai⁶, T B Liang⁷, J P Sheng⁸

Affiliations

¹ Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China.
² Department of Medical Oncology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China.
³ Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, China.
⁴ Infinity Scope Biotechnology Co., Ltd., Hangzhou 311200, China.
⁵ College of Computer Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing 210000, China. Electronic address: shaowei20022005@nuaa.edu.cn.
⁶ Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China. Electronic address: shirleybai@zju.edu.cn.
⁷ Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China. Electronic address: liangtingbo@zju.edu.cn.
⁸ Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310002, China; Zhejiang University Cancer Center, Zhejiang University, Hangzhou 310002, China. Electronic address: shengjp@zju.edu.cn.

PMID: 37922312
DOI: 10.1016/j.celrep.2023.113364

Abstract

Erythro-myeloid progenitors of the yolk sac that originates during early embryo development has been suggested to generate tissue-resident macrophage, mast cell, and even endothelial cell populations from fetal to adult stages. However, the heterogeneity of erythro-myeloid progenitors (EMPs) is not well characterized. Here, we adapt single-cell RNA sequencing to dissect the heterogeneity of EMPs and establish several fate-mapping tools for each EMP subset to trace the contributions of different EMP subsets. We identify two primitive and one definitive EMP subsets from the yolk sac. In addition, we find that primitive EMPs are decoupled from definitive EMPs. Furthermore, we confirm that primitive and definitive EMPs give rise to microglia and other tissue-resident macrophages, respectively. In contrast, only Kit⁺ Csf1r^- primitive EMPs generate endothelial cells transiently during early embryo development. Overall, our results delineate the contribution of yolk sac EMPs more clearly based on the single-cell RNA sequencing (scRNA-seq)-guided fate-mapping toolkit.

Keywords: CP: Developmental biology; CP: Stem cell research.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Lineage
Endothelial Cells*
Hematopoiesis / genetics
Microglia
Myeloid Progenitor Cells
Sequence Analysis, RNA
Yolk Sac*