Cardiac cellular diversity and functionality in cardiac repair by single-cell transcriptomics

Wei Chen; Chuling Li; Yijin Chen; Jianping Bin; Yanmei Chen

doi:10.3389/fcvm.2023.1237208

Cardiac cellular diversity and functionality in cardiac repair by single-cell transcriptomics

Front Cardiovasc Med. 2023 Oct 18:10:1237208. doi: 10.3389/fcvm.2023.1237208. eCollection 2023.

Authors

Wei Chen^{1

2}, Chuling Li^{1

2}, Yijin Chen^{1

2}, Jianping Bin^{1

2}, Yanmei Chen^{1

2

3}

Affiliations

¹ Department of Cardiology, State Key Laboratory of Organ Failure Research, Nanfang Hospital, Southern Medical University, Guangzhou, China.
² Guangdong Provincial Key Laboratory of Cardiac Function and Microcirculation, Guangzhou, China.
³ Department of Cardiology, Ganzhou People's Hospital, Ganzhou, China.

Abstract

Cardiac repair after myocardial infarction (MI) is orchestrated by multiple intrinsic mechanisms in the heart. Identifying cardiac cell heterogeneity and its effect on processes that mediate the ischemic myocardium repair may be key to developing novel therapeutics for preventing heart failure. With the rapid advancement of single-cell transcriptomics, recent studies have uncovered novel cardiac cell populations, dynamics of cell type composition, and molecular signatures of MI-associated cells at the single-cell level. In this review, we summarized the main findings during cardiac repair by applying single-cell transcriptomics, including endogenous myocardial regeneration, myocardial fibrosis, angiogenesis, and the immune microenvironment. Finally, we also discussed the integrative analysis of spatial multi-omics transcriptomics and single-cell transcriptomics. This review provided a basis for future studies to further advance the mechanism and development of therapeutic approaches for cardiac repair.

Keywords: cardiac repair; left ventricular remodeling; myocardial infarction; single-cell transcriptomics; therapeutic approach.

Publication types

Review

Grants and funding

This work was supported by grants to Yanmei Chen from the National Natural Science Foundation of China (no. 81970239). GuangDong Basic and Applied Basic Research Foundation (grant 2023A1515010381 and grant 2022A1515220013 to YC), Natural Science Foundation of Jiangxi Province (grant 20232ACB216003 to YC).