Microbial nitrogen mineralization is slightly affected by conversion from farmland to apple orchards in thick loess deposits

Abstract

Organic nitrogen mineralization, indispensable to soil carbon and nitrogen cycles, is the largest contributor to nitrate reservoirs in deep vadose zones. The microbial nitrogen mineralization (MNM) within deep soils, particularly in regions with intensive agricultural activities and thick soil horizons, has been largely disregarded. As such, this study aims to address this knowledge gap by investigating the chiA-harboring microbial structure and network within nine 10-m profiles beneath cultivated farmland and two apple orchards. The results showed that apple orchards, compared to farmland, had considerable water deficit and nitrogen accumulation within deeper soil layers due to well-developed root systems and the overuse of chemical fertilizers. However, the chiA-harboring microbial diversity, composition, and abundance all exhibited significant variations with soil depths rather than being influenced by different land use types. Moreover, the diversity indices and gene abundances decreased with soil depths, and the related soil microbes included 19 phyla, 29 classes, 72 orders, 114 families, and 197 genera, with Actinobacteria and Proteobacteria being the two major bacterial phyla. The microbial co-occurrence network was simper beneath apple orchards. The chiA-harboring microorganisms within deep unsaturated zones were greatly influenced by the depth-dependent soil nutrients, such as total nitrogen, organic carbon, and available potassium. The limited plant root biomass and the inhibitory effects of dried soil layers both restricted the availability of carbon sources, which further interfered with the MNM processes within deep soils insignificantly. Therefore, despite the considerable plant-induced ecohydrological consequences, the depth-dependent MNM processes were slightly affected after the transformation from farmland to apple orchards within thick loess deposits. This study offers crucial insights into microbial dynamics of the deep biosphere, thereby contributing to our understanding of depth-dependent biogeochemical cycles within global deep unsaturated zones.

Keywords: Amplicon sequencing; Co-occurrence network; Depth-dependence; Microbial nitrogen mineralization (MNM); Quantitative PCR (qPCR).