A Combination of CPI-0610 and SAHA Induces Apoptosis through STAT3 and p38 Signalling Pathways in Diffuse Large B-cell Lymphoma Cells

Linyan Xu; Jun Jiao; Mengdi Liu; Yuanyuan Qin; Meng Zhang; Dongmei Yan; Kailin Xu; Wei Sang

doi:10.2174/0109298673244868231017043517

A Combination of CPI-0610 and SAHA Induces Apoptosis through STAT3 and p38 Signalling Pathways in Diffuse Large B-cell Lymphoma Cells

Curr Med Chem. 2023 Oct 27. doi: 10.2174/0109298673244868231017043517. Online ahead of print.

Authors

Linyan Xu¹, Jun Jiao¹, Mengdi Liu¹, Yuanyuan Qin¹, Meng Zhang¹, Dongmei Yan², Kailin Xu¹, Wei Sang²

Affiliations

¹ Xuzhou Medical University Blood Diseases Institute Xuzhou China.
² Xuzhou Medical University Department of Hematology Xuzhou China.

PMID: 37916635
DOI: 10.2174/0109298673244868231017043517

Abstract

Background: Although immunotherapies have greatly improved diffuse large B-cell lymphoma (DLBCL) prognosis, a proportion of patients remain to be relapsed or refractory. Therefore, the identification of novel therapeutic targets and drugs is urgently required. Inhibition of the bromodomain and extra-terminal (BET) proteins has been a promising therapeutic strategy for various haematologic cancers. CPI-0610 is a potent and selective BET inhibitor. The effects of CPI-0610 in DLBCL cells have not been reported yet.

Aims: The aim of this study was to assess the effects of CPI-0610 in DLBCL and its underlying mechanisms.

Methods: DLBCL cells were treated with CPI-0610, followed by measuring cell viability, cell cycle, apoptosis, autophagy, and specific cell signaling pathways. Moreover, immunodeficient mice were engrafted with SUDHL2 cells and then treated with CPI-0610 for analysis of tumor burden. We also analyzed the synergistic effect of CPI-0610 with histone deacetylase inhibitor suberoylanilide hydroxamic acid.

Results: The present study demonstrated that CPI-0610 displayed cell cytotoxicity by arresting the G1 cell cycle and inducing endogenous and exogenous apoptotic pathways. Additionally, CPI-0610 decreased BRD4 and c-Myc expressions and affected MAPK, JAK/STAT, and AKT signalling pathways in human DLBCL cells. An in vivo experiment exhibited that CPI-0610 decreased the primary tumour growth of the DLBCL xenograft model. Furthermore, the use of CPI-0610 in combination with suberoylanilide hydroxamic acid exhibited a specific synergistic effect in inducing apoptosis through the regulation of STAT3 and p38.

Conclusion: Targeting BET may be an effective therapeutic strategy and potentiated by a combination with histone deacetylase inhibition in DLBCL.

Keywords: BRD4; CPI-0610; SAHA; SATA3; apoptosis; p38.