IMF deposition ceRNA network analysis and functional study of HIF1a in yak

Mengning Luo; Hui Wang; Jun Zhang; Kangzhu Yixi; Shi Shu; Changqi Fu; Jincheng Zhong; Wei Peng

doi:10.3389/fvets.2023.1272238

IMF deposition ceRNA network analysis and functional study of HIF1a in yak

Front Vet Sci. 2023 Oct 17:10:1272238. doi: 10.3389/fvets.2023.1272238. eCollection 2023.

Authors

Mengning Luo¹, Hui Wang¹, Jun Zhang², Kangzhu Yixi¹, Shi Shu², Changqi Fu², Jincheng Zhong¹, Wei Peng²

Affiliations

¹ Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Sichuan Province and Ministry of Education, Southwest Minzu University, Chengdu, China.
² Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, China.

Abstract

The concentration of intramuscular fat (IMF) is a crucial determinant of yak meat quality. However, the molecular mechanisms that regulate IMF in yak remain largely elusive. In our study, we conducted transcriptome sequencing on the longissimus dorsi muscle tissues of yaks with varying IMF contents. We then filtered differentially expressed genes (DEGs), microRNAs (DEMs), and long non-coding RNAs (DELs) to elucidate potential regulatory pathways of adipogenesis in yaks. Overall, our research sheds light on an array of potential mRNAs and noncoding RNAs implicated in IMF deposition and elaborates on the role of HIF1α in yaks. These findings contribute valuable insights that can serve as a guide for further research into the molecular mechanisms governing IMF deposition.

Keywords: HIF1α; IMF; RNA-seq; adipogenesis; ceRNA; yak.

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the National Natural Science Foundation of China (no. 31902153), the Qinghai Science and Technology Program, China (no. 2022-NK-110), the Program of National Beef Cattle and Yak Industrial Technology System (no. CARS-37), and the Fundamental Research Funds for the Central Universities, Southwest Minzu University (2022NYXXS049). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.