Elevated high-mannose N-glycans hamper endometrial decidualization

Siyi Chen; Aihui Zhang; Na Li; Hongpan Wu; Yaqi Li; Shuai Liu; Qiu Yan

doi:10.1016/j.isci.2023.108170

Elevated high-mannose N-glycans hamper endometrial decidualization

iScience. 2023 Oct 14;26(11):108170. doi: 10.1016/j.isci.2023.108170. eCollection 2023 Nov 17.

Authors

Siyi Chen¹, Aihui Zhang¹, Na Li¹, Hongpan Wu¹, Yaqi Li¹, Shuai Liu¹, Qiu Yan¹

Affiliation

¹ Liaoning Provincial Core Lab of Glycobiology and Glycoengineering, College of Basic Medical Science, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China.

Abstract

Decidualization of endometrial stromal cells is a hallmark of endometrial receptivity for embryo implantation, and dysfunctional decidualization is associated with pregnancy failure. Protein glycosylation is an important posttranslational modification that affects the structure and function of glycoproteins. Our results showed that high-mannose epitopes were elevated in the decidual tissues of miscarriage patients compared with early pregnant women by Lectin microarray. Furthermore, the level of mannosyl-oligosaccharide α-1,2 mannosidase IA (MAN1A1), a key enzyme for high-mannose glycan biosynthesis, was decreased in the decidual tissues of miscarriage patients. Screening of lncRNAs showed that lncNEAT1 level was increased in the serum and decidua of miscarriage patients, and negatively correlated with MAN1A1 expression. The results also revealed that specific binding of lncNEAT1 with nucleophosmin (NPM1)-SP1 transcription complex inhibited MAN1A1 expression and hampered endometrial decidualization and embryo implantation potential. The study suggests the new insights into the function of high-mannose glycans/MAN1A1 modification during endometrial decidualization.

Keywords: Biochemistry; Molecular biology; Physiology.