So far mating type determination in Neurospora crassa requires test crosses with strains of known mating type. We present a simple, quick, and reliable polymerase chain reaction-based method for mating type determination in N. crassa.
Keywords: Neurospora crassa; PCR-based mating type identification; mating type.
© 2023 The Authors. Journal of Basic Microbiology published by Wiley-VCH GmbH.