A NitroPure Nitrocellulose Membrane-Based Grapevine Virus Sampling Kit: Development and Deployment to Survey Japanese Vineyards and Nurseries

Viruses. 2023 Oct 17;15(10):2102. doi: 10.3390/v15102102.

Abstract

We developed a NitroPure Nitrocellulose (NPN) membrane-based method for sampling and storing grapevine sap for grapevine virus detection. We devised an efficient nucleic acid extraction method for the NPN membrane, resulting in 100% amplification success for grapevine leafroll-associated virus 2 (GLRaV2) and 3 (GLRaV3), grapevine rupestris stem pitting-associated virus (GRSPaV), grapevine virus A, grapevine virus B, and grapevine red blotch virus (GRBV). This method also allowed the storage of recoverable nucleic acid for 18 months at room temperature. We created a sampling kit to survey GLRaV2, GLRaV3, and GRBV in Japanese vineyards. We tested the kits in the field in 2018 and then conducted mail-in surveys in 2020-2021. The results showed a substantial prevalence of GLRaV3, with 48.5% of 132 sampled vines being positive. On the other hand, only 3% of samples tested positive for GLRaV2 and none for GRBV.

Keywords: GLRaV2; GLRaV3; GRBV; NitroPure nitrocellulose; grapevine viruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Collodion
  • Farms
  • Geminiviridae*
  • Nucleic Acids*
  • Plant Diseases
  • Vitis*

Substances

  • Collodion
  • Nucleic Acids

Supplementary concepts

  • Grapevine leafroll-associated virus 2

Grants and funding

This research was funded by Japan Grants-in-Aid for Scientific Research (aka Kakenhi), grant number 19K06049.