Complete Mitochondrial Genome Characterization of Schrankia costaestrigalis (Insecta: Erebidae: Hypenodinae) and Its Phylogenetic Implication

Genes (Basel). 2023 Sep 26;14(10):1867. doi: 10.3390/genes14101867.

Abstract

The pinion-streaked snout Schrankia costaestrigalis is a new potato pest that has recently been recorded in China. In this study, we analyzed the complete mitochondrial genome of S. costaestrigalis. The results revealed the mitogenome (GenBank: OQ181231) to occur as a circular DNA molecule of 16,376 bp with 51.001% AT content, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 1 control region. Notably, the PCGs exhibited typical ATN (Met) start codons, including cox1, which deviated from the usual CGA start codon observed in other lepidopteran mitogenomes, and followed the conventional TAN stop codons. The 22 tRNA genes demonstrated the ability to form a cloverleaf structure, with the exception of trnS1-NCU, which lacked the DHU arm present in other Erebidae mitogenomes. Additionally, conserved motifs like "ATAGA + poly-T (19 bp) stretch" and five microsatellite-like elements (TA) were identified in the AT-rich region. The phylogenetic trees revealed that the Hypenodinae subfamily forms an independent lineage closely related to Erebinae and Catocalinae. The comprehensive mitogenome of S. costaestrigalis will greatly enhance future studies focused on the molecular classification and phylogenetic understanding of the Hypenodinae subfamily within the larger family Erebidae.

Keywords: Hypenodinae; Schrankia costaestrigalis; mitochondrial genome; phylogenetic analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Codon, Terminator
  • Genome, Mitochondrial*
  • Moths* / genetics
  • Phylogeny
  • RNA, Transfer / chemistry
  • RNA, Transfer / genetics

Substances

  • RNA, Transfer
  • Codon, Terminator

Grants and funding

This research was funded by the Guangxi Natural Science Foundation under Grant No. 2020GXNSFBA297162, the fund of Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests under Grant No.20-065-30-ST-01 and the Foundational Research Fund of Guangxi Academy of Agricultural Sciences under Grant No. 2022YM04.