Advancing Nanoscale Science: Synthesis and Bioprinting of Zeolitic Imidazole Framework-8 for Enhanced Anti-Infectious Therapeutic Efficacies

Muhammad Saqib Saif; Murtaza Hasan; Ayesha Zafar; Muhammad Mahmood Ahmed; Tuba Tariq; Muhammad Waqas; Riaz Hussain; Amna Zafar; Huang Xue; Xugang Shu

doi:10.3390/biomedicines11102832

Advancing Nanoscale Science: Synthesis and Bioprinting of Zeolitic Imidazole Framework-8 for Enhanced Anti-Infectious Therapeutic Efficacies

Biomedicines. 2023 Oct 18;11(10):2832. doi: 10.3390/biomedicines11102832.

Authors

Muhammad Saqib Saif¹, Murtaza Hasan^{2

3}, Ayesha Zafar⁴, Muhammad Mahmood Ahmed⁵, Tuba Tariq¹, Muhammad Waqas², Riaz Hussain⁶, Amna Zafar⁵, Huang Xue³, Xugang Shu³

Affiliations

¹ Faculty of Chemical and Biological Science, Department of Biochemistry, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
² Faculty of Chemical and Biological Science, Department of Biotechnology, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
³ School of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
⁴ School of Engineering, Royal Melbourne Institute of Technology (RMIT) University, 24 La Trobe Street, Melbourne, VIC 3001, Australia.
⁵ Faculty of Chemical and Biological Science, Department of Bioinformatics, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
⁶ Faculty of Chemical and Biological Science, Department of Veterinary Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.

Abstract

Bacterial infectious disorders are becoming a major health problem for public health. The zeolitic imidazole framework-8 with a novel Cordia myxa extract-based (CME@ZIF-8) nanocomposite showed variable functionality, high porosity, and bacteria-killing activity against Staphylococcus aureus, and Escherichia coli strains have been created by using a straightforward approach. The sizes of synthesized zeolitic imidazole framework-8 (ZIF-8) and CME@ZIF-8 were 11.38 nm and 12.44 nm, respectively. Prepared metal organic frameworks have been characterized by gas chromatography-mass spectroscopy, Fourier transform spectroscopy, UV-visible spectroscopy, X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. An antibacterial potential comparison between CME@ZIF-8 and zeolitic imidazole framework-8 has shown that CME@ZIF-8 was 31.3%, 28.57%, 46%, and 47% more efficient than ZIF-8 against Staphylococcus aureus and 43.7%, 42.8%, 35.7%, and 70% more efficient against Escherichia coli, while it was 31.25%, 33.3%, 46%, and 46% more efficient than the commercially available ciprofloxacin drug against Staphylococcus aureus and 43.7%, 42.8%, 35.7%, and 70% more efficient against Escherichia coli, respectively, for 750, 500, 250, and 125 μg mL^-1. Minimum inhibitory concentration values of CME@ZIF-8 for Escherichia coli and Staphylococcus aureus were 15.6 and 31.25 μg/mL respectively, while the value of zeolitic imidazole framework-8 alone was 62.5 μg/mL for both Escherichia coli and Staphylococcus aureus. The reactive oxygen species generated by CME@ZIF-8 destroys the bacterial cell and its organelles. Consequently, the CME@ZIF-8 nanocomposites have endless potential applications for treating infectious diseases.

Keywords: CME@ZIF-8; antibacterial; metal organic frameworks MOFs; nanocomposite.

Grants and funding

The Islamia University Bahawalpur, Pakistan, National Research Program for University (NRPU) for Higher Education Commission, Pakistan (Grant ID: 9458).