Generation and characterization of iPS cell line (SYSUSHi002-A) from PBMCs of an AML patient with TP53 mutation

Lihua Yuan; Yaqun Wang; Rongli Xie; Dongjun Lin; Xiaobo Wang

doi:10.1016/j.scr.2023.103230

Generation and characterization of iPS cell line (SYSUSHi002-A) from PBMCs of an AML patient with TP53 mutation

Stem Cell Res. 2023 Dec:73:103230. doi: 10.1016/j.scr.2023.103230. Epub 2023 Oct 18.

Authors

Lihua Yuan¹, Yaqun Wang², Rongli Xie², Dongjun Lin², Xiaobo Wang³

Affiliations

¹ Pediatric Purgery Department, The University of Hong Kong Shenzhen Hospital, Shenzhen, Guangdong Province 518000, China.
² Hematology Department, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong Province, 518107, China.
³ Hematology Department, The Seventh Affiliated Hospital of Sun Yat-Sen University, Shenzhen, Guangdong Province, 518107, China. Electronic address: wangxiaobo@sysush.com.

PMID: 37890328
DOI: 10.1016/j.scr.2023.103230

Abstract

We have generated an iPSCs line (IPS-AML2-C3, SYSUSHi002-A) from AML cells of a 71-year-old male Acute Myeloid Leukaemia patient with TP53 gene mutation (TP53: c.824G > A, p.Cys275Tyr) using episomal plasmids encoding the factors OCT4, SOX2, KLF4, L-MYC and human miR-302. The IPS-AML2-C3 (SYSUSHi002-A) iPSC line displayed typical embryonic stem cell-like morphology, carried the TP53 gene mutation, expressed several pluripotent stem cell makers, retained normal karyotype (46, XY), and was capable of forming three germ layer cells in vitro.

Publication types

Case Reports

MeSH terms

Aged
Cell Differentiation
Cell Line
Humans
Induced Pluripotent Stem Cells* / metabolism
Kruppel-Like Factor 4
Leukemia, Myeloid, Acute* / genetics
Leukemia, Myeloid, Acute* / metabolism
Male
Mutation / genetics
Transcription Factors / genetics
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Protein p53 / metabolism

Substances

Transcription Factors
Kruppel-Like Factor 4
TP53 protein, human
Tumor Suppressor Protein p53