Comprehensive insights into herbicide resistance mechanisms in weeds: a synergistic integration of transcriptomic and metabolomic analyses

Madhab Kumar Sen; Soham Bhattacharya; Rohit Bharati; Katerina Hamouzová; Josef Soukup

doi:10.3389/fpls.2023.1280118

Comprehensive insights into herbicide resistance mechanisms in weeds: a synergistic integration of transcriptomic and metabolomic analyses

Front Plant Sci. 2023 Oct 11:14:1280118. doi: 10.3389/fpls.2023.1280118. eCollection 2023.

Authors

Madhab Kumar Sen¹, Soham Bhattacharya¹, Rohit Bharati², Katerina Hamouzová¹, Josef Soukup¹

Affiliations

¹ Department of Agroecology and Crop Production, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Suchdol, Czechia.
² Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Suchdol, Czechia.

Abstract

Omics techniques, including genomics, transcriptomics, proteomics, and metabolomics have smoothed the researcher's ability to generate hypotheses and discover various agronomically relevant functions and mechanisms, as well as their implications and associations. With a significant increase in the number of cases with resistance to multiple herbicide modes of action, studies on herbicide resistance are currently one of the predominant areas of research within the field of weed science. High-throughput technologies have already started revolutionizing the current molecular weed biology studies. The evolution of herbicide resistance in weeds (particularly via non-target site resistance mechanism) is a perfect example of a complex, multi-pathway integration-induced response. To date, functional genomics, including transcriptomic and metabolomic studies have been used separately in herbicide resistance research, however there is a substantial lack of integrated approach. Hence, despite the ability of omics technologies to provide significant insights into the molecular functioning of weeds, using a single omics can sometimes be misleading. This mini-review will aim to discuss the current progress of transcriptome-based and metabolome-based approaches in herbicide resistance research, along with their systematic integration.

Keywords: abiotic stresses; herbicide resistance; metabolomics; omics technologies; transcriptomic regulation.

Publication types

Review

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by grant from the Long-term conceptual development of research organization [The Ministry of Education, Youth and Sports (Czech Republic)]. Rohit Bharati was supported by the Internal Grant Agency, grant number 20233105, Faculty of Tropical AgriSciences, Czech University of Life Sciences in Prague.