Proteomic analysis of leaves and roots during drought stress and recovery in Setaria italica L

Hui Gao; Weina Ge; Lin Bai; Ting Zhang; Ling Zhao; Jingshi Li; Jiangjie Shen; Ningwei Xu; Haoshan Zhang; Genping Wang; Xiaohu Lin

doi:10.3389/fpls.2023.1240164

Proteomic analysis of leaves and roots during drought stress and recovery in Setaria italica L

Front Plant Sci. 2023 Oct 11:14:1240164. doi: 10.3389/fpls.2023.1240164. eCollection 2023.

Authors

Hui Gao^{1

2}, Weina Ge³, Lin Bai⁴, Ting Zhang², Ling Zhao², Jingshi Li¹, Jiangjie Shen¹, Ningwei Xu⁵, Haoshan Zhang², Genping Wang², Xiaohu Lin¹

Affiliations

¹ Hebei Key Laboratory of Crop Stress Biology, Department of Life Science and Technology, College of Marine Resources and Environment, Hebei Normal University of Science and Technology, Qinhuangdao, Hebei, China.
² Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals(Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/Key Laboratory of Minor Cereal Crops of Hebei Province, Shijiazhuang, China.
³ College of Life Sciences, North China University of Science and Technology, Tangshan, China.
⁴ State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Institute of Biomedical Sciences, Fudan University, Shanghai, China.
⁵ College of Landscape and Tourism, Hebei Agricultural University, Baoding, China.

Abstract

Drought is a major environmental factor that limits agricultural crop productivity and threatens food security. Foxtail millet is a model crop with excellent abiotic stress tolerance and is consequently an important subject for obtaining a better understanding of the molecular mechanisms underlying plant responses to drought and recovery. Here the physiological and proteomic responses of foxtail millet (cultivar Yugu1) leaves and roots to drought treatments and recovery were evaluated. Drought-treated foxtail millet exhibited increased relative electrolyte leakage and decreased relative water content and chlorophyll content compared to control and rewatering plants. A global analysis of protein profiles was evaluated for drought-treated and recovery treatment leaves and roots. We also identified differentially abundant proteins in drought and recovery groups, enabling comparisons between leaf and root tissue responses to the conditions. The principal component analysis suggested a clear distinction between leaf and root proteomes for the drought-treated and recovery treatment plants. Gene Ontology enrichment and co-expression analyses indicated that the biological responses of leaves differed from those in roots after drought and drought recovery. These results provide new insights and data resources to investigate the molecular basis of tissue-specific functional responses of foxtail millet during drought and recovery, thereby significantly informing crop breeding.

Keywords: Setaria italica L; drought stress; leaf; proteomic; root.

Grants and funding

This research was funded by the Scientific Research Foundation of Hebei Normal University of Science and Technology, grant number 2018YB003, the Science and Technology Research Project of Higher Education Institutions of Hebei Province, grant number QN2020154, the National Key R&D Program of China, grant number 2018YFD1000705, the HAAFS Basic Science and Technology Contract Project, grant number HBNKY-BGZ-02, and Key R&D Projects in Hebei Province, grant number 22326314D.