The roles of miRNAs in adult skeletal muscle satellite cells

Pieter Jan Koopmans; Ahmed Ismaeel; Katarzyna Goljanek-Whysall; Kevin A Murach

doi:10.1016/j.freeradbiomed.2023.10.403

The roles of miRNAs in adult skeletal muscle satellite cells

Free Radic Biol Med. 2023 Nov 20;209(Pt 2):228-238. doi: 10.1016/j.freeradbiomed.2023.10.403. Epub 2023 Oct 24.

Authors

Pieter Jan Koopmans¹, Ahmed Ismaeel², Katarzyna Goljanek-Whysall³, Kevin A Murach⁴

Affiliations

¹ Exercise Science Research Center, Molecular Muscle Mass Regulation Laboratory, Department of Health, Human Performance, and Recreation, University of Arkansas, Fayetteville, AR, 72701, USA; Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, 72701, USA.
² Department of Physiology, College of Medicine, University of Kentucky, Lexington, KY, 40506, USA.
³ School of Medicine, College of Medicine, Nursing, and Health Sciences, University of Galway, Galway, Ireland.
⁴ Exercise Science Research Center, Molecular Muscle Mass Regulation Laboratory, Department of Health, Human Performance, and Recreation, University of Arkansas, Fayetteville, AR, 72701, USA; Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, 72701, USA. Electronic address: kmurach@uark.edu.

PMID: 37879420
PMCID: PMC10911817 (available on 2024-11-20)
DOI: 10.1016/j.freeradbiomed.2023.10.403

Abstract

Satellite cells are bona fide muscle stem cells that are indispensable for successful post-natal muscle growth and regeneration after severe injury. These cells also participate in adult muscle adaptation in several capacities. MicroRNAs (miRNAs) are post-transcriptional regulators of mRNA that are implicated in several aspects of stem cell function. There is evidence to suggest that miRNAs affect satellite cell behavior in vivo during development and myogenic progenitor behavior in vitro, but the role of miRNAs in adult skeletal muscle satellite cells is less studied. In this review, we provide evidence for how miRNAs control satellite cell function with emphasis on satellite cells of adult skeletal muscle in vivo. We first outline how miRNAs are indispensable for satellite cell viability and control the phases of myogenesis. Next, we discuss the interplay between miRNAs and myogenic cell redox status, senescence, and communication to other muscle-resident cells during muscle adaptation. Results from recent satellite cell miRNA profiling studies are also summarized. In vitro experiments in primary myogenic cells and cell lines have been invaluable for exploring the influence of miRNAs, but we identify a need for novel genetic tools to further interrogate how miRNAs control satellite cell behavior in adult skeletal muscle in vivo.

Keywords: Myogenesis; Senescence; Stem cells; Transcriptomics; myomiRs.

Publication types

Review
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / genetics
MicroRNAs* / genetics
MicroRNAs* / metabolism
Muscle, Skeletal / metabolism
Satellite Cells, Skeletal Muscle* / metabolism
Stem Cells / metabolism

Substances

MicroRNAs

Abstract

Publication types

MeSH terms

Substances

Grants and funding