The JAK/STAT/NF-κB signaling pathway can be regulated by rosemary essential oil, thereby providing a potential treatment for DNCB-induced in mice

Biomed Pharmacother. 2023 Dec:168:115727. doi: 10.1016/j.biopha.2023.115727. Epub 2023 Oct 24.

Abstract

Objective: The purpose of this study was to investigate the mechanism through which rosemary essential oil treats atopic dermatitis.

Methods: A dinitrochlorobenzene (DNCB)-induced atopic dermatitis mouse model was established and treated with low (1%), medium (2%), and high (4%) doses of Rosmarinus officinalis essential oil (EORO). Serum levels of interleukin (IL)-6 and tumor necrosis factor-alpha (TNF-α) in each group were determined using enzyme-linked immunosorbent assay (ELISA). Skin tissues were stained with hematoxylin-eosin and toluidine blue. We used network pharmacology and molecular docking techniques to verify the biological activity of essential proteins and their corresponding compounds in the pathway. Gas chromatography-mass spectrometry (GC-MS) was used for metabolomics analysis and multivariate statistical analysis of mouse serum to screen differential metabolites and metabolic pathway analysis. Protein expression of p-JAK1, CD4+ cells, and IL-4 in the skin tissue was detected by immunohistochemistry analysis. Protein levels of STAT3, p-STAT3, P65, and p-P65 in damaged skin tissues were detected using western blotting.

Result: The skin of mice in the model group showed different degrees of erythema, dryness, scratches, epidermal erosion and shedding, and crusting. After treatment, the serum levels of IL-6 and TNF-α in EORO group were significantly decreased, and the expression of p-JAK1,CD4 + cells, IL-4, p-P65 / P65 and p-STAT3 / STAT3 proteins in skin tissues were decreased.

Conclusion: EORO can effectively improve DNCB-induced AD-like skin lesions in mice by regulating the JAK/STAT/NF-κB signaling pathway, thereby reducing the production of downstream arachidonic acid metabolites, inhibiting skin inflammation, and restoring epidermal barrier function.

Keywords: Atopic dermatitis; Metabolomics; Molecular docking; Rosemary essential oil; Weight coefficient Network Pharmacology.

MeSH terms

  • Animals
  • Cytokines / metabolism
  • Dermatitis, Atopic* / drug therapy
  • Dinitrochlorobenzene / pharmacology
  • Interleukin-4 / metabolism
  • Interleukin-6 / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Molecular Docking Simulation
  • NF-kappa B / metabolism
  • Oils, Volatile* / pharmacology
  • Oils, Volatile* / therapeutic use
  • Rosmarinus*
  • Signal Transduction
  • Skin / metabolism
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Cytokines
  • Dinitrochlorobenzene
  • Interleukin-4
  • Interleukin-6
  • NF-kappa B
  • Oils, Volatile
  • Rosmarinus officinalis essential oil (EORO)
  • Tumor Necrosis Factor-alpha
  • rosemary oil