A rapid and high-throughput system for the detection of transgenic products based on LAMP-CRISPR-Cas12a

Hua Liu; Xiuwen Hu; Haijuan Zeng; Chuan He; Fang Cheng; Xueming Tang; Jinbin Wang

doi:10.1016/j.crfs.2023.100605

A rapid and high-throughput system for the detection of transgenic products based on LAMP-CRISPR-Cas12a

Curr Res Food Sci. 2023 Oct 4:7:100605. doi: 10.1016/j.crfs.2023.100605. eCollection 2023.

Authors

Hua Liu¹, Xiuwen Hu², Haijuan Zeng¹, Chuan He¹, Fang Cheng³, Xueming Tang⁴, Jinbin Wang¹

Affiliations

¹ Institute of Biotechnology Research, Shanghai Academy of Agricultural Sciences, Key Laboratory of Agricultural Genetics and Breeding, 2901 Beidi Road, Shanghai, 201106, China.
² College of Food Sciences and Technology, Shanghai Ocean University, 999 Huancheng Road Shanghai, 200120, China.
³ Institute of Natural Sciences, Shanghai Jiao Tong University, Shanghai, 200240, China.
⁴ School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Abstract

With the increasing acreage of genetically modified crops worldwide, rapid and efficient detection technologies have become very important for the regulation and screening of GM organisms. We constructed a method based on loop-mediated isothermal amplification (LAMP), CRISPR-Cas12a and lateral flow assay (LAMP-CRISPR-Cas12a-LFA). It is an intuitive, sensitive and specific fluorescence detection and test strip system to detect CP4-EPSPS and Cry1Ab/Ac genes in field screening. The LAMP-CRISPR-Cas12a-LFA method has a limit of detection (LOD) of 100 copies based on lateral flow test strips after optimization of the conditions with screened specific primers, and the entire detection process can be completed within 1 h at 61 °C. The system was used to evaluate field test samples and showed high reproducibility after testing products containing CP4-EPSPS and Cry1Ab/Ac genes, and both were detectable. The LAMP-CRISPR-Cas12a-LFA method established in this paper functions as a rapid field detection method. It requires only one portable thermostatic instrument, which renders it compatible with the rapid detection of field samples and useable at experimental workstations, in law enforcement field work, and in local inspection and quarantine departments.

Keywords: CRISPR-Cas12a; GM organisms; LAMP; Rapid detection.