Ultrasound-assisted and subcritical water extraction techniques for maximal recovery of phenolic compounds from raw ginger herbal dust toward in vitro biological activity investigation

Mirjana Sulejmanović; Nataša Milić; Ioannis Mourtzinos; Nataša Nastić; Anastasia Kyriakoudi; Jovana Drljača; Senka Vidović

doi:10.1016/j.foodchem.2023.137774

Ultrasound-assisted and subcritical water extraction techniques for maximal recovery of phenolic compounds from raw ginger herbal dust toward in vitro biological activity investigation

Food Chem. 2024 Mar 30;437(Pt 1):137774. doi: 10.1016/j.foodchem.2023.137774. Epub 2023 Oct 14.

Authors

Mirjana Sulejmanović¹, Nataša Milić², Ioannis Mourtzinos³, Nataša Nastić⁴, Anastasia Kyriakoudi⁵, Jovana Drljača⁶, Senka Vidović⁷

Affiliations

¹ Department of Pharmaceutical Engineering, Faculty of Technology Novi Sad, University of Novi Sad, Boulevard Cara Lazara 1, 21000 Novi Sad, Serbia. Electronic address: mirjana.sulejmanovic@uns.ac.rs.
² Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia. Electronic address: NATASA.MILIC@mf.uns.ac.rs.
³ Laboratory of Food Chemistry - and Biochemistry, Department of Food Science and Technology, Faculty of Agriculture, Aristotle University, Thessaloniki 541 24, Greece. Electronic address: mourtzinos@agro.auth.gr.
⁴ Department of Pharmaceutical Engineering, Faculty of Technology Novi Sad, University of Novi Sad, Boulevard Cara Lazara 1, 21000 Novi Sad, Serbia. Electronic address: natasa.nastic@uns.ac.rs.
⁵ Laboratory of Food Chemistry - and Biochemistry, Department of Food Science and Technology, Faculty of Agriculture, Aristotle University, Thessaloniki 541 24, Greece. Electronic address: anakyria@agro.auth.gr.
⁶ Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia. Electronic address: jovana.drljaca@mf.uns.ac.rs.
⁷ Department of Pharmaceutical Engineering, Faculty of Technology Novi Sad, University of Novi Sad, Boulevard Cara Lazara 1, 21000 Novi Sad, Serbia. Electronic address: senka.vidovic@uns.ac.rs.

PMID: 37866343
DOI: 10.1016/j.foodchem.2023.137774

Abstract

This study examined the impact of two green extraction techniques in order to maximize the usage and recovery of phenolic compounds from the by-product of the filter tea industry, the so-called ginger herbal dust. The main phenolic compounds extraction was performed by ultrasound-assisted extraction (UAE) with the sonication amplitude ranging from 20% to 100%, and the subcritical water extraction (SWE), with the temperature ranging from 120 °C to 220 °C. All obtained extracts were characterized in terms of extraction yield, total phenolic content (TPC), and 6-ginerol, 6-shogaol, and 8-ginerol contents using RP-HPLC-DAD. Based on the results, we selected the extract obtained from raw ginger herbal dust using a sonication amplitude of 100% for further biological investigation of the cytotoxic effect on short- and long-term cell viability on liver and pancreatic cancer cells. This extract contained high TPC concentration, and 6-gingerol (44.57 mg/gDE), 8-gingerol (8.62 mg/gDE), and 6-shogaol (6.92 mg/gDE).

Keywords: Cytotoxic activity; Ginger herbal dust; Green extraction technologies; Subcritical water extraction; Ultrasound-assisted extraction; Waste utilization.

MeSH terms

Phenols / pharmacology
Plant Extracts / pharmacology
Water*
Zingiber officinale*

Substances

shogaol
Water
Plant Extracts
Phenols