Relative contribution of ammonia-oxidizing bacteria and denitrifying fungi to N2O production during rice straw composting with biochar and biogas residue amendments

Xu Lin; Naif Abdullah Al-Dhabi; Fanghong Li; Nanyi Wang; Hua Peng; Anwei Chen; Genyi Wu; Jiachao Zhang; Lihua Zhang; Hongli Huang; Binghua Yan; Lin Luo; Wangwang Tang

doi:10.1016/j.biortech.2023.129891

Relative contribution of ammonia-oxidizing bacteria and denitrifying fungi to N₂O production during rice straw composting with biochar and biogas residue amendments

Bioresour Technol. 2023 Dec:390:129891. doi: 10.1016/j.biortech.2023.129891. Epub 2023 Oct 18.

Authors

Xu Lin¹, Naif Abdullah Al-Dhabi², Fanghong Li³, Nanyi Wang¹, Hua Peng⁴, Anwei Chen¹, Genyi Wu⁵, Jiachao Zhang⁶, Lihua Zhang¹, Hongli Huang¹, Binghua Yan¹, Lin Luo¹, Wangwang Tang⁷

Affiliations

¹ College of Environment and Ecology, Hunan Agricultural University, 410128, China; Yuelu Mountain Laboratory, Hunan Agricultural University Area, Changsha 410000, Hunan, China.
² Department of Botany and Microbiology, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia.
³ State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment of the PR China, Guangzhou 510655, China.
⁴ Hunan Institute of Agro-Environment and Ecology, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
⁵ College of Environment and Ecology, Hunan Agricultural University, 410128, China.
⁶ College of Environment and Ecology, Hunan Agricultural University, 410128, China; Yuelu Mountain Laboratory, Hunan Agricultural University Area, Changsha 410000, Hunan, China. Electronic address: jiachao.zhang@163.com.
⁷ College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.

PMID: 37863336
DOI: 10.1016/j.biortech.2023.129891

Abstract

Nitrous oxide (N₂O) production is associated with ammonia-oxidizing bacteria (amoA-AOB) and denitrifying fungi (nirK-fungi) during the incorporation of biochar and biogas residue composting. This research examined the relative contribution of alterations in the abundance, diversity and structure of amoA-AOB and nirK-fungi communities on N₂O emission by real-time PCR and sequence processing. Results showed that N₂O emissions showed an extreme relation with the abundance of amoA-AOB (rs = 0.584) while giving credit to nirK-fungi (rs = 0.500). Nitrosomonas and Nitrosospira emerged as the dominant genera driving ammoxidation process. Biogas residue changed the community structure of AOB by altering Nitrosomonadaceae proportion and physiological capacity. The denitrification process, primarily governed by nirK-fungi, served as a crucial pathway for N₂O production, unveiling the pivotal mechanism of biochar to suppress N₂O emissions. C/N and NH₄⁺-N were identified as significant parameters influencing the distribution of nirK-fungi, especially Micromonospora, Halomonas and Mesorhizobium.

Keywords: Biochar; Biogas residue; Composting; N(2)O emission; amoA-AOB; nirK-fungi.

MeSH terms

Ammonia / metabolism
Betaproteobacteria* / metabolism
Biofuels
Composting*
Denitrification
Nitrification
Nitrous Oxide / analysis
Oryza* / metabolism
Oxidation-Reduction
Soil / chemistry
Soil Microbiology

Substances

biochar
Ammonia
Biofuels
Soil
Nitrous Oxide