Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H2O2

Shiwen Hu; Pan Wang; Jianhong Ke; Junmin Hui; Cun Wang; Jing Luo; Shaocheng Chen

doi:10.3892/etm.2023.12216

Protective effect of Peucedanum praeruptorum Dunn extract on oxidative damage of LLC‑PK1 cells induced by H₂O₂

Exp Ther Med. 2023 Sep 21;26(5):517. doi: 10.3892/etm.2023.12216. eCollection 2023 Nov.

Authors

Shiwen Hu^{1

2}, Pan Wang³, Jianhong Ke⁴, Junmin Hui², Cun Wang², Jing Luo¹, Shaocheng Chen^{1

2}

Affiliations

¹ Chongqing Field Scientific Observation and Research Station for Authentic Traditional Chinese Medicine in the Three Gorges Reservoir Area, Chongqing University of Education, Chongqing 400067, P.R. China.
² College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China.
³ Department of Traumatology, Chongqing University Central Hospital/Chongqing Emergency Medical Center, Chongqing 400013, P.R. China.
⁴ Corn Research Institute, Chongqing Academy of Agricultural Sciences, Chongqing 401329, P.R. China.

Abstract

Peucedanum praeruptorum Dunn extract (PPDE) is a well-known treatment used in traditional Chinese medicines, where it is most commonly used to treat coughs and symptoms such as headaches and fever. In the present study, the antioxidant capacity of PPDE in vitro was determined by scavenging experiments using DPPH, ABTS⁺·, ·OH, and ·O₂^-. The cell survival rate was determined by MTT assay. The MDA, SOD, CAT, GSH, and GSH-Px content were determined by colorimetry assays. The expression levels of antioxidant genes SOD, CAT, GSH, and GSH-Px were assessed by reverse transcription-quantitative PCR. HPLC was used to identify the PPDE components. The results suggested that PPDE had scavenging effects on DPPH, ABTS, hydroxyl, and superoxide anion radicals in a concentration-dependent manner; H₂O₂ treatment resulted in oxidative stress in LLC-PK1 cells, and the degree of injury of LLC-PK1 cells following PPDE treatment was improved, which was positively correlated with its concentration. Peucedanum praeruptorum Dunn extract treatment reduced the content of MDA and increased the content of CAT, SOD1, GSH, and GSH-Px. The mRNA expression levels of antioxidant genes detected by quantitative PCR were consistent with changes in CAT, SOD, GSS, and GSH-Px. Additionally, the trend in CAT, SOD1, GSH, and GSS protein expression levels was also consistent at the mRNA level. PPDE was found to consist of isochlorogenic acid C, myricetin, baicalin, luteolin, and kaempferol. Therefore, PPDE, which was formed of products derived from natural substances, functioned in the inhibition of oxidative damage. The present study aimed to obtain a better understanding of the traditional Chinese medicine Peucedanum praeruptorum Dunn and preliminarily elucidate its antioxidant mechanism at the cellular level. Further animal or human experiments are required to verify the antioxidant effects of PPDE for further development and utilization.

Keywords: H2O2; Peucedanum praeruptorum Dunn; oxidative damage; pig renal epithelial LLC-PK1 cells.

Grants and funding

Funding: This research was funded by the Science and Technology Project of Chongqing Education Commission (grant no. KJZD-M201901601) and Chongqing University Innovation Research Group Project (grant no. CXQTP20033).