AGouTI-Flexible Annotation of Genomic and Transcriptomic Intervals

PLoS Comput Biol. 2023 Oct 18;19(10):e1011527. doi: 10.1371/journal.pcbi.1011527. eCollection 2023 Oct.

Abstract

The recent development of high-throughput workflows in genomics and transcriptomics revealed that efficient annotation of such results is essential for researchers to draw conclusions from obtained results. Although some tools are available, their functionality is limited. Here, we present AGouTI-a universal tool for flexible annotation of any genomic or transcriptomic coordinates using known genomic features deposited in different publicly available databases in the form of GTF or GFF files. In contrast to currently available tools, AGouTI is designed to provide a flexible selection of genomic features overlapping or adjacent to annotated intervals and can be used on custom column-based text files obtained from different data analysis pipelines. Although providing many unique options, AGouTI is straightforward in installation and usage, enabling effortless integration into existing data analysis workflows.

MeSH terms

  • Animals
  • Dasyproctidae*
  • Genome / genetics
  • Genomics / methods
  • Molecular Sequence Annotation
  • Software
  • Transcriptome / genetics

Grants and funding

This work has been supported by the National Science Centre (Poland, 2011/03/D/NZ2/03304 to M.Ż) and National Centre for Research and Development (Poland, POWR.0302.00-00-I006/17 to J.G.K.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.