Oxidative stress and cryoresistance of sturgeon sperm: A review

Abstract

Currently, the majority of sturgeons are relict fishes of high economic value yet endangered with extinction. Cryopreservation of sperm has great potential in fish farming and conservation, but the problem of low cryoresistance of sturgeon sperm has not yet been solved. The goal of this work was to review current literature data on the causes of low tolerance of sturgeon sperm to cryodamage. The influence of cryopreservation on the parameters of physiology and metabolism of sturgeon sperm (morphology and fine ultrastructure, mobility and fertilization ability, integrity of the plasma membrane, protein, lipid and metabolite profiles, antioxidant status, DNA damage), as well as on biomarkers of oxidative stress (lipids peroxidation levels and carbonyl derivatives of proteins) is discussed. Since the development of oxidative stress is an important mechanism of sperm cryodamage induction, the review presents the literature on the role of oxygen-derived species in damage of sturgeon reproductive cells caused by cryopreservation. Particular attention is paid to the system of antioxidant protection of sturgeon seminal plasma and spermatozoa, represented by antioxidant enzymes and low molecular weight antioxidants capable of utilizing various reactive forms of oxygen and nitrogen. The review discusses the results of lipidomic and proteomic studies of sturgeon sperm, which made it possible to obtain new data on the lipid composition of cell membranes, to detect proteins involved in the protection of sturgeon spermatozoa from oxidative damage during cryopreservation. This review presents the use of «omics» technology to elucidate the mechanism of cryodamage in sturgeon sperm. Additionally, the review summarizes information on the unique anatomical, morphological, biochemical, and physiological features of sturgeon sperm, which may be associated with low cryoresistance of sturgeon, in order to establish prospects for further research on improving the methods of the conservation of sperm of these threatened species.

Keywords: Antioxidant system; Cryodamage; Cryopreservation; Family Acipenseridae; Reactive oxygen species; Spermatozoa.