Huge quantities of agricultural waste are available annually in Saudi Arabia, which are not well exploited, leading to large environmental pollution and health problems. The use of different proportions of tomato waste compost (TWC) mixed with commercial peat-moss as a natural, economical, cheap and environmentally friendly fertilizer for fertilizing vegetables is very important, therefore, this study is concerned with investigating and evaluating the dry matter content (DM) and the accumulated heavy metal contents (HMs) in the root and leaf seedlings tissues of cucumber and summer squash plants as a result of using this fertilizer. Five ratios of mixtures (vermiculite: peat moss: TWC by weight) used in the experiment, and the TWC in the mixture represents 0, 5, 10, 15 and 20% (by wieght). The results indicated that, the DM contents in the root and leaf tissues were relatively close between cucumber and summer squash seedlings. However, they were higher in the root than in leaf tissues, particularly under 20% TWC. A TWC application ratio of 20% reduced concentrations of the most HMs in the two vegetable species seedlings due to high EC and pH levels. The amounts of HMs in vegetable species were not high, which proves the that transfer of HMs from the TWC substrate to the seedlings is determined chiefly by metal bioavailability and by a vegetable species. DM, mineral and nutritional quality of vegetable seedlings showed that substrates containing 5% and 10% TWC appeared to be the most suitable growing medium for economic vegetable seedlings; and it is recommended for use in the nursery production. Implications: The idea of this research revolves around the use of different proportions of tomato waste compost in fertilizing cucumber and summer squash seedlings and investigate the effect of this compost on dry matter (DM) and heavy metal (HMs) contents in the root and leaf tissues of the seedlings, and fruit quality of cucumber and summer squash plants.